1.13.11.3: protocatechuate 3,4-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 3,4-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.3

1.13.11.3

catecholate

1,2-dioxygenase

intradiol

4-hydroxybenzoate

3,4-dihydroxybenzoate

beta-ketoadipate

ring-cleavage

gentisate

beta-carboxy-cis,cis-muconate

enol-lactone

hydroxyquinol

orville

lipscomb

fuscum

cis-muconate

4-methylcatechols

3,5-di-tert-butylcatechol

analysis

environmental protection

degradation

Reaction

Synonyms

3,4-PCase, 3,4-PCD, 3,4-PCDase, 3,4-POD, EC 1.13.1.3, EC 1.99.2.3, More, oxygenase, protocatechuate 3,4-di-, P3,4DO, P3,4O enzyme, P34O, PCA 3,4-dioxygenase, PcaG, PcaH, PcaHG, PCD, protocatchetuate 3,4-dioxygenase, protocatechuate 3,4-dioxygenase, protocatechuate oxygenase, protocatechuic 3,4-dioxygenase, protocatechuic 3,4-oxygenase, protocatechuic acid oxidase

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.11 With incorporation of two atoms of oxygen

1.13.11.3 protocatechuate 3,4-dioxygenase

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Results	in table
9	Activating Compound
6616	AA Sequence
5	Application
1	CAS Registry Number
15	Cloned(Commentary)
12	Crystallization (Commentary)
9	Expression
11	General Information
8	General Stability
3	IC50 Value
135	Inhibitors
3	kcat/KM [mM/s]
42	Ki Value [mM]
58	KM Value [mM]
1	Localization
21	Metals/Ions
42	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
123	Organism
12	Pathway
386	PDB ID
24	pH Optimum
9	pH Range
4	pH Stability
1	Posttranslational Modification
21	Protein Variants
31	Purification (Commentary)
5	Reaction
3	Reaction Type
89	Reference
5	Source Tissue
30	Specific Activity [micromol/min/mg]
10	Storage Stability
174	Substrates and Products (Substrate)
23	Subunits
50	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Temperature Range [°C]
16	Temperature Stability [°C]
41	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.13.11.3 - protocatechuate 3,4-dioxygenase

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150000

2 entries

189900

Pseudomonas oleovorans

gel filtration

190000

gel filtration

198000

202000

meniscus depletion

204000

sedimentation equilibrium

439504

21502

Hydrogenophaga palleronii

x * 27097 + x * 21502, deduced from nucleotide sequence

439513

21900

Sagittula stellata

x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits

439510

22000

Acinetobacter lwoffii

x * 26000 + x * 22000, 2D-PAGE

439514

220000

Rhizobium leguminosarum

gel filtration

439499

22280

Pseudomonas putida

alpha,beta, 2 * 22280 + 2 * 26630, holoenzyme probably contains 6 alpha2,beta2 tetramers, amino acid sequence

439493

22500

Brevibacterium fuscum

alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE

439484, 439503

23000

2 entries

23300

Azotobacter vinelandii

alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE

439501

25500

Azotobacter vinelandii

alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE

439501

26000

Acinetobacter lwoffii

x * 26000 + x * 22000, 2D-PAGE

439514

26500

3 entries

26630

Pseudomonas putida

alpha,beta, 2 * 22280 + 2 * 26630, holoenzyme probably contains 6 alpha2,beta2 tetramers, amino acid sequence

439493

26700

Sagittula stellata

x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits

439510

27097

Hydrogenophaga palleronii

x * 27097 + x * 21502, deduced from nucleotide sequence

439513

29000

2 entries

315000

Brevibacterium fuscum

analytical ultracentrifugation

439484, 439503

34300

Pseudomonas oleovorans

trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE

725655

40000

Brevibacterium fuscum

alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE

439484, 439503

480000

2 entries

500000

Rhizobium sp. LMB-1

A0A193DXA9; A0A193DXP2

gel filtration

741660

510000

Azotobacter vinelandii

gel filtration

590000

ultracentrifugation

63230

analytical ultracentrifugation

763959

677000

Acinetobacter calcoaceticus

sedimentation velocity analysis

439500

700000

Pseudomonas aeruginosa

diffusion data

439483

783100

Pseudomonas aeruginosa

calculated

665455

97832

Pseudomonas aeruginosa

8 * 97832, calculated

665455