1.13.11.3: protocatechuate 3,4-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 3,4-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.3

1.13.11.3

catecholate

1,2-dioxygenase

intradiol

4-hydroxybenzoate

3,4-dihydroxybenzoate

beta-ketoadipate

ring-cleavage

gentisate

beta-carboxy-cis,cis-muconate

enol-lactone

hydroxyquinol

orville

lipscomb

fuscum

cis-muconate

4-methylcatechols

3,5-di-tert-butylcatechol

analysis

environmental protection

degradation

Reaction

Synonyms

3,4-PCase, 3,4-PCD, 3,4-PCDase, 3,4-POD, EC 1.13.1.3, EC 1.99.2.3, More, oxygenase, protocatechuate 3,4-di-, P3,4DO, P3,4O enzyme, P34O, PCA 3,4-dioxygenase, PcaG, PcaH, PcaHG, PCD, protocatchetuate 3,4-dioxygenase, protocatechuate 3,4-dioxygenase, protocatechuate oxygenase, protocatechuic 3,4-dioxygenase, protocatechuic 3,4-oxygenase, protocatechuic acid oxidase

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.11 With incorporation of two atoms of oxygen

1.13.11.3 protocatechuate 3,4-dioxygenase

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Results	in table
9	Activating Compound
6616	AA Sequence
5	Application
1	CAS Registry Number
15	Cloned(Commentary)
12	Crystallization (Commentary)
9	Expression
11	General Information
8	General Stability
3	IC50 Value
135	Inhibitors
3	kcat/KM [mM/s]
42	Ki Value [mM]
58	KM Value [mM]
1	Localization
21	Metals/Ions
42	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
123	Organism
12	Pathway
386	PDB ID
24	pH Optimum
9	pH Range
4	pH Stability
1	Posttranslational Modification
21	Protein Variants
31	Purification (Commentary)
5	Reaction
3	Reaction Type
89	Reference
5	Source Tissue
30	Specific Activity [micromol/min/mg]
10	Storage Stability
174	Substrates and Products (Substrate)
23	Subunits
50	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Temperature Range [°C]
16	Temperature Stability [°C]
41	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.13.11.3 - protocatechuate 3,4-dioxygenase

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-20°C, stable for several years

Brevibacterium fuscum

439484

0-3°C, several weeks, no loss in activity

Pseudomonas fluorescens

439481

25°C, free enzyme, 30 days, 61% loss of activity

Pseudomonas putida

743818

25°C, immobilized enzyme, 30 days, 56% loss of activity

Pseudomonas putida

743818

4°C, free enzyme, 30 days, 59% loss of activity

Pseudomonas putida

743818

4°C, immobilized enzyme, 30 days, 56% loss of activity

Pseudomonas putida

743818

4°C, pH 8.5, 6 months

Acinetobacter calcoaceticus

439500

4°C, stability of free and the immobilized protocatechuate 3,4-dioxygenase in phosphate buffer containing 50 mM, pH 7.0, for free enzyme or immobilized enzyme in calcium alginate, and 40mM, pH 8.0, for enzyme immobilized on glyoxyl agarose, immobilized enzyme shows 9.93% of its initial activity after 28 days of storage, while the free enzyme becomes inactive

Stenotrophomonas maltophilia

I0DHJ0; I0DHJ1

742046

5°C, long periods, no loss in activity

stable only 7 days