1.13.11.3: protocatechuate 3,4-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 3,4-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.3

Reaction

3,4-dihydroxybenzoate
+
O2
=
3-carboxy-cis,cis-muconate

Synonyms

3,4-PCase, 3,4-PCD, 3,4-PCDase, 3,4-POD, EC 1.13.1.3, EC 1.99.2.3, More, oxygenase, protocatechuate 3,4-di-, P3,4DO, P3,4O enzyme, P34O, PCA 3,4-dioxygenase, PcaG, PcaH, PcaHG, protocatchetuate 3,4-dioxygenase, protocatechuate 3,4-dioxygenase, protocatechuate oxygenase, protocatechuic 3,4-dioxygenase, protocatechuic 3,4-oxygenase, protocatechuic acid oxidase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.3 protocatechuate 3,4-dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.3 - protocatechuate 3,4-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for several years
-
0-3°C, several weeks, no loss in activity
-
25°C, free enzyme, 30 days, 61% loss of activity
-
25°C, immobilized enzyme, 30 days, 56% loss of activity
-
4°C, free enzyme, 30 days, 59% loss of activity
-
4°C, immobilized enzyme, 30 days, 56% loss of activity
-
4°C, pH 8.5, 6 months
-
4°C, stability of free and the immobilized protocatechuate 3,4-dioxygenase in phosphate buffer containing 50 mM, pH 7.0, for free enzyme or immobilized enzyme in calcium alginate, and 40mM, pH 8.0, for enzyme immobilized on glyoxyl agarose, immobilized enzyme shows 9.93% of its initial activity after 28 days of storage, while the free enzyme becomes inactive
5°C, long periods, no loss in activity
-
stable only 7 days
-