1.13.11.31: arachidonate 12-lipoxygenase

This is an abbreviated version!
For detailed information about arachidonate 12-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.31

Reaction

arachidonate
+
O2
=
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate

Synonyms

(12R)-lipoxygenase, (12R)-LOX, 11R-lipoxygenase, 11R-LOX, 12(R)-lipoxygenase, 12(S)-lipoxygenase, 12-hLO, 12-lipoxygenase, 12-LO, 12-LOX, 12/15 lipoxygenase, 12/15-lipoxygenase, 12/15-lipoxygenases, 12/15-LO, 12/15-LOX, 12/15LO, 12DELTA-lipoxygenase, 12LO, 12R-lipoxygenase, 12R-LOX, 12R-LOX-2, 12S-lipoxygenase, 15-lipoxygenase, 15-LO, 2-lipoxygenase, 2/15-lipoxygenase, ALOX 12, ALOX 12/15, ALOX12, Alox12b, Alox15, ALOX15B, ALOX5, arachidonate 12/15-lipoxygenase, C-12 lipoxygenase, cardiac 12/15-LOX, DELTA 12-lipoxygenase, epidermal-type lipoxygenase, hp-12LOX, human platelet 12-lipoxygenase, leukocyte-type 12-lipoxygenase, leukocyte-type 12-LOX, leukocyte-type 12/15-lipoxygenase, leukocyte-type lipoxygenase, leukotriene A4 synthase M, lipoxygenase 12, LOX, LOX-12, LTA4 synthase, oxylipin-producing enzyme, oygenase, arachidonate 12-lip-, P-12LOX, p12-LO, p12-LOX, platelet 12-lipoxygenase, platelet-type 12(S)-lipoxygenase, platelet-type 12-human lipoxygenase, platelet-type 12-lipoxygenase, platelet-type 12-LOX, platelet-type 12LO, Platelet-type lipoxygenase 12, zf12-LOX

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.31 arachidonate 12-lipoxygenase

Cloned

Cloned on EC 1.13.11.31 - arachidonate 12-lipoxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
12/15LO stably overexpressed in mouse J774 macrophages
-
enzyme is cloned into the expression vectors pSE280, pSE380, and pSE420. PSE380 yields the highest level of 12-lipoxygenase activity, expression in Escherichia coli Top10 cells
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expressed from pET-20b(+) plasmid in Escherichia coli Rosetta 2 (DE3) cells
expressed in Escherichia coli
expressed in Escherichia coli and Sf9 insect cells
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expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
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expressed with hexa-His tags
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expression in CHO and in neuro-2 A neuroblastoma cells both stably expressing human APP carrying the K670N/M671L Swedish mutation, expression analysis
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expression in Escherichia coli
expression in HEK 293 cells
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expression in HEK-293 cells by using the eukaryotic expression vector pcDNA3
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expression in insect cells using a baculovirus expression vector, expression as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site
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expression of His-tagged enzyme
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expression of His-tagged wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
expression of isozyme 12-hLO as His-tagged enzyme
expression of N-terminally His6-tagged enzyme
full-length cDNA for 12/15LO subcloned into vector pACCMVpLpA. Bone marrow-derived macrophages infected at a multiplicity of infection of 50 for 48 h with recombinant adenoviral vector AdLO to overexpress 12/15LO
gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme
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gene alox15, expression analysis and genotyping of wild-type and iron-deficient mutant rats, gene alox15 doesnot respond to iron, transcription regulation via cytokines and growth factors, overview
gene ALOX15, located on the short arm of chromosome 11
genotyping, overexpression of His-tagged mutant W633X and wild-type Alox12b proteins in COS7 cells
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue)
overexpressed as N-terminally, His6-tagged protein
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rat beta INS-1 cells are transduced with adeno-associated virus vector ACSM12-LO, that encodes mouse leukocyte type 12-lipoxygenase
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recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
recombinant overexpression in J774A.1 cells
sequence comparisons and phylogenetic analysis
transgenic mice overexpressing 12/15-LOX in cardiomyocytes
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two cDNA clones encoding the platelet-type and the leukocyte-type isoforms expressed in COS-7 cells
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