1.13.11.37: hydroxyquinol 1,2-dioxygenase

This is an abbreviated version, for detailed information about hydroxyquinol 1,2-dioxygenase, go to the full flat file.

Reaction

Benzene-1,2,4-triol
+
O2
=
maleylacetate

Synonyms

1,2-HQD, Ar 1,2-HQD, cphA-1, cphA-2, cphA-I, HQD, hydroxyquinol 1,2-dioxygenase, hydroxyquinol-1,2-dioxygenase, hydroxyquinon dioxygenase, More, NpcC, oxygenase, hydroxyquinol 1,2-di-, oxygenase, hydroxyquinol di-, PdcC, PnpC, PnpG

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.37 hydroxyquinol 1,2-dioxygenase

Cloned

Cloned on EC 1.13.11.37 - hydroxyquinol 1,2-dioxygenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cphA-1 from Arthrobacter chloropenolicus A6 is cloned into the vectro pCRT7/CT-TOPO, the construct is transformed into CL21(DE3)/pLysS cells.; cphA-2 from Arthrobacter chloropenolicus A6 is cloned into the vectro pCRT7/CT-TOPO, the construct is transformed into CL21(DE3)/pLysS cells.
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells; gene cphA-I, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
expression in Escherichia coli BL21
-
gene pnpC, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-