1.13.11.37: hydroxyquinol 1,2-dioxygenase

This is an abbreviated version, for detailed information about hydroxyquinol 1,2-dioxygenase, go to the full flat file.

Reaction

Benzene-1,2,4-triol
+
O2
=
maleylacetate

Synonyms

1,2-HQD, Ar 1,2-HQD, cphA-1, cphA-2, cphA-I, HQD, hydroxyquinol 1,2-dioxygenase, hydroxyquinol-1,2-dioxygenase, hydroxyquinon dioxygenase, More, NpcC, oxygenase, hydroxyquinol 1,2-di-, oxygenase, hydroxyquinol di-, PdcC, PnpC, PnpG

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.37 hydroxyquinol 1,2-dioxygenase

Purification

Purification on EC 1.13.11.37 - hydroxyquinol 1,2-dioxygenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Ni-NTA column chromatography; recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration by 51.7-65.2fold from Escherichia coli strain BL21(DE3)
Ni-NTA column chromatography
-
recombinant 1,2-HQD
recombinant His-tagged enzyme 37.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-