1.13.11.37: hydroxyquinol 1,2-dioxygenase

This is an abbreviated version, for detailed information about hydroxyquinol 1,2-dioxygenase, go to the full flat file.

Reaction

Benzene-1,2,4-triol
+
O2
=
maleylacetate

Synonyms

1,2-HQD, Ar 1,2-HQD, cphA-1, cphA-2, cphA-I, HQD, hydroxyquinol 1,2-dioxygenase, hydroxyquinol-1,2-dioxygenase, hydroxyquinon dioxygenase, More, NpcC, oxygenase, hydroxyquinol 1,2-di-, oxygenase, hydroxyquinol di-, PdcC, PnpC, PnpG

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.37 hydroxyquinol 1,2-dioxygenase

Specific Activity

Specific Activity on EC 1.13.11.37 - hydroxyquinol 1,2-dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.37
-
substrate hydroxyquinol
0.45
-
substrate catechol
5.5
recombinant enzyme, crude enzyme extract, substrate 4-chlorocatechol, pH 7.5, 25C
7.06
-
reactivated with 0.01 mM Fe2+
9.3
-
purified recombinant His-tagged enzyme, pH 5.0, 45C
15.3
recombinant enzyme, crude enzyme extract, substrate hydroxyquinol, pH 7.5, 25C
284.6
51.7fold purified enzyme, with 4-chlorocatechol as substrate, at pH 7.5 and 25C; purified recombinant enzyme, substrate 4-chlorocatechol, pH 7.5, 25C
391
62.5fold purified enzyme, with catechol as substrate, at pH 7.5 and 25C; purified recombinant enzyme, substrate catechol , pH 7.5, 25C
479.5
purified recombinant enzyme, substrate 3-methylcatechol, pH 7.5, 25C
479.6
65.2fold purified enzyme, with 3-methylcatechol as substrate, at pH 7.5 and 25C
795.6
52fold purified enzyme, with hydroxyquinol as substrate, at pH 7.5 and 25C; purified recombinant enzyme, substrate hydroxyquinol, pH 7.5, 25C
additional information