1.13.11.41: 2,4'-dihydroxyacetophenone dioxygenase
This is an abbreviated version!
For detailed information about 2,4'-dihydroxyacetophenone dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.41
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1.13.11.41
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cupin
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alcaligenes
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homotetramer
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formic
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4-hydroxybenzoic
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ketone
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chymotrypsinolysis
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tridentate
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biomimetic
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nonhaem
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non-heme
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2-hydroxyacetophenone
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equiv
- 1.13.11.41
-
cupin
- alcaligenes
-
homotetramer
-
formic
-
4-hydroxybenzoic
- ketone
-
chymotrypsinolysis
-
tridentate
-
biomimetic
-
nonhaem
-
non-heme
- 2-hydroxyacetophenone
-
equiv
Reaction
Synonyms
(4-hydroxybenzoyl)methanol oxygenase, DAD, DHAP dioxygenase
ECTree
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Metals Ions
Metals Ions on EC 1.13.11.41 - 2,4'-dihydroxyacetophenone dioxygenase
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Fe2+
Iron
additional information
Fe2+
ha catalytic function and is coordinated by His76, His78, and His116, structure and catalytic mechanism, overview
Fe2+
one non-heme iron per enzyme subunit. The catalytic iron is coordinated by three histidine residues, H76, H78 and H114 in strands II and VII, within a buried active-site cavity, and an additional ligand
Fe2+
the enzyme contains 1.69 mol of non-heme iron per mol of enzyme, His77, His79, His115, and Glu96 in the cupin fold are putative metal ligands, Fe2+ weakly activates the enzyme
Fe2+
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anaerobic reconstitution of the purified enzyme with five equivalents of Fe2+ results in an increase in activity of 7% which increases to 13% upon overnight incubation in air
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the enzyme first binds to iron(III) and subsequently transfers an electron to the iron center, reducing it to iron(II)
additional information
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incubation of apoenzyme with Ca2+, Mg2+, Mn2+, Ni2+, or Zn2+ results in no effect on the background activity after either 2 h or overnight