1.13.11.5: homogentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about homogentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.5

Reaction

homogentisate
+
O2
=
4-Maleylacetoacetate

Synonyms

EC 1.13.1.5, EC 1.99.2.5, ElHDO, HgD, HGDO, HGO, HGOa, HGOb, HmgA, homogentisate 1,2 dioxygenase, homogentisate 1,2-dioxygenase, homogentisate dioxygenase, homogentisate oxidase, homogentisate oxygenase, homogentisate phytyl-transferase, homogentisic acid 1,2-dioxygenase, homogentisic acid oxidase, homogentisic acid oxygenase, homogentisic oxygenase, homogentisicase, HPT, HTO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.5 homogentisate 1,2-dioxygenase

Engineering

Engineering on EC 1.13.11.5 - homogentisate 1,2-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R378G
A122D
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
C1273A
-
naturally occuring mutation, the mutation is involved in alkaptonuria
E42A
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
G161R
-
naturally occuring mutation in the HGD gene, resulting in a specific genotype appearing in a Hungarian population, originating from Slovakia, with alkaptonuria, phenotype overview
G360R
-
active site mutation in exon 13
H80Q
-
the mutant shows slightly reduced specific activity compared to the wild type enzyme
I216T
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
K57N
-
active site mutation in exon 3
M368V
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
R225H
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
S189I
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
T1046G
-
naturally occuring mutation, the mutation is involved in alkaptonuria
T533G
-
naturally occuring mutation, the mutation is involved in alkaptonuria
T847C
-
naturally occuring mutation, the mutation is involved in alkaptonuria
W60G
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
Y62C
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
H288Q
site-directed mutagenesis, the mutant shows a 75fold reduction in kcat compared to the wild-type enzyme
Y346F
site-directed mutagenesis, replacement of Y346 by phenylalanine decreases the affinity for homogentisate more than 60fold and reduces the apparent kcat 20fold resulting in a decrease of the specificity constant by three orders of magnitude compared to the wild-type enzyme
H288Q
-
site-directed mutagenesis, the mutant shows a 75fold reduction in kcat compared to the wild-type enzyme
-
Y346F
-
site-directed mutagenesis, replacement of Y346 by phenylalanine decreases the affinity for homogentisate more than 60fold and reduces the apparent kcat 20fold resulting in a decrease of the specificity constant by three orders of magnitude compared to the wild-type enzyme
-
additional information