1.13.11.5: homogentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about homogentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.5

Reaction

homogentisate
+
O2
=
4-Maleylacetoacetate

Synonyms

EC 1.13.1.5, EC 1.99.2.5, ElHDO, HgD, HGDO, HGO, HGOa, HGOb, HmgA, homogentisate 1,2 dioxygenase, homogentisate 1,2-dioxygenase, homogentisate dioxygenase, homogentisate oxidase, homogentisate oxygenase, homogentisate phytyl-transferase, homogentisic acid 1,2-dioxygenase, homogentisic acid oxidase, homogentisic acid oxygenase, homogentisic oxygenase, homogentisicase, HPT, HTO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.5 homogentisate 1,2-dioxygenase

Inhibitors

Inhibitors on EC 1.13.11.5 - homogentisate 1,2-dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
inactivation in the absence of O2 due to formation of a white precipitate corresponding to apoprotein
2,2'-dipyridyl
2-hydroxyphenylacetate
-
strong competitive inhibition with respect to homogentisate
2-mercaptoethanol
-
-
3,4-Dihydroxyphenylacetate
-
1 mM, 48% inhibition, substrate analogue
3-chlorohomogentisate
-
inactivation in the presence of O2 due to formation of a white precipitate corresponding to apoprotein
3-hydroxyphenylacetate
-
competitive inhibition with respect to homogentisate
ascorbate
-
strong inhibitory effect, but stabilizes
ascorbic acid
-
38% inhibition at pH 6.2
benzoquinoneacetic acid
-
-
CaCl2
-
0.001 mM, 56% inhibition
Co2+
-
incubation with Fe2+ plus Co2+ in equimolar concentrations inhibits
Cu2+
-
incubation with Fe2+ plus Cu2+ in equimolar concentrations inhibits
cyanide
cysteic acid
-
weak inhibition
cysteine
-
inhibition not reversed by glutathione
cystine
-
inhibition not reversed by glutathione
diphosphate
-
-
FeSO4
-
0.25 mM, 42% inhibition
glutathione
-
reduced glutathione slightly inhibits
Hg2+
-
inhibition reversed by glutathione
HgCl2
-
20 mM, complete inhibition
Lewisite
-
inhibits by competing with Fe2+ for a common enzymic binding site
Methylmercuric bromide
-
inhibits by competing with Fe2+ for a common enzymic binding site
NaCl
-
0.001 mM, 63% inhibition
Ni2+
-
incubation with Fe2+ plus Ni2+ in equimolar concentrations inhibits
p-chloromercuribenzoate
p-hydroxyphenylacetic acid
-
80 mM, complete inhibition, substrate analogue
p-hydroxyphenylpyruvate
-
1 mM, 68% inhibition, substrate analogue
phosphate
-
-
sulfhydryl reagents
-
strong inhibition
Superoxide dismutase
-
inhibits the reaction indicating the involvement of superoxide anions in the dioxygenase reaction
-
Zn2+
-
incubation with Fe2+ plus Zn2+ in equimolar concentrations inhibits
additional information
-
not inhibited by Ca2+ and Mn2+
-