1.13.11.5: homogentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about homogentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.5

Reaction

homogentisate
+
O2
=
4-Maleylacetoacetate

Synonyms

EC 1.13.1.5, EC 1.99.2.5, ElHDO, HgD, HGDO, HGO, HGOa, HGOb, HmgA, homogentisate 1,2 dioxygenase, homogentisate 1,2-dioxygenase, homogentisate dioxygenase, homogentisate oxidase, homogentisate oxygenase, homogentisate phytyl-transferase, homogentisic acid 1,2-dioxygenase, homogentisic acid oxidase, homogentisic acid oxygenase, homogentisic oxygenase, homogentisicase, HPT, HTO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.5 homogentisate 1,2-dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.5 - homogentisate 1,2-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM potassium phosphate buffer, pH 7.0, crude extracts, 5 months, 25% loss of activity
-
-20°C, in absence of Fe2+, about 3 days, stable
-
-20°C, pH 6.0-7.2, in absence of Fe2+, several weeks, stable
-
-80, 20 mM Hepps, pH 8.0, 100 mM NaCl, 4 months, no loss of activity
-
4°C, 20 mM Tris-Cl pH 7.8, 0.5 M NaCl, 0.3 M imidazole, 24 h, loses two third of its activity