1.13.11.50: acetylacetone-cleaving enzyme

This is an abbreviated version!
For detailed information about acetylacetone-cleaving enzyme, go to the full flat file.

Reaction

Synonyms

acetylacetone dioxygenase, acetylacetone-cleaving enzyme, b-diketone dioxygenase, beta-diketone dioxygenase, cupin-type dioxygenase, diketone cleaving dioxygenase, diketone cleaving enzyme, diketone dioxygenase, diketone-cleaving dioxygenase, diketone-cleaving enzyme, DKDO, Dke1, oxygenase, b-diketone di-, pentane-2,4-dione hydrolase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.50 acetylacetone-cleaving enzyme

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Results	in table
14	AA Sequence
1	Application
1	CAS Registry Number
7	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
3	General Information
1	General Stability
11	Inhibitors
15	KM Value [mM]
1	Localization
22	Metals/Ions
11	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
15	Organism
4	PDB ID
4	pH Optimum
2	pH Range
25	Protein Variants
6	Purification (Commentary)
4	Reaction
5	Reaction Type
15	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
45	Substrates and Products (Substrate)
6	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
31	Turnover Number [1/s]

Purification

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Purification on EC 1.13.11.50 - acetylacetone-cleaving enzyme

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Acinetobacter johnsonii

-

636327, 636328

affinity column chromatography

Acinetobacter johnsonii

-

675466

purified, concentrated enzyme is dialyzed twice against 2 mM EDTA in 20 mM Tris/HCl (pH 7.5) to strip off iron, incubation in 2 mM metal ion as sulfate salt plus ascorbate to prevent Fe2+ oxidation, unbound metal ions are removed by 3 cycles of gel filtration using nucleic acid purification column

Acinetobacter johnsonii

Q8GNT2

696107

recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography

Acinetobacter johnsonii

-

725553

Sepharose Q fast flow column chromatography Superdex 200 gel filtration, Resource Q column chromatography, Nap-5 column gel filtration, and Phenyl Sepharose HP chromatography

Acinetobacter johnsonii

-

671683