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1.13.11.55: sulfur oxygenase/reductase

This is an abbreviated version!
For detailed information about sulfur oxygenase/reductase, go to the full flat file.

Word Map on EC 1.13.11.55

Reaction

4 sulfur + 4 H2O +

O2
= 2 hydrogen sulfide + 2 sulfite

Synonyms

AaSOR, SAMN00768000_1627, SAMN00768000_1798, SOR, SOR protein, SOR-AT, sulfur oxygenase, sulfur oxygenase reductase, sulfuroxygenasereductase, sulphur oxygenase reductase, TpSOR, TPY_0405

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.55 sulfur oxygenase/reductase

Purification

Purification on EC 1.13.11.55 - sulfur oxygenase/reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
heat treatment, DEAE-52 anion exchange and Sephadex G-200 chromatography
-
one-step procedure over Strep-Tactin columns
purified by gel filtration
recombinant protein, by heat treatment and chromatography steps with four different columns
-
recombinant wild-type and mutant enzymes from Escherichia coli
single-step purification of the proteins is obtained via fused His or Strep tags
solubilized recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies by nickel affinity chromatography, and refolded by a four-step dialysis
-
sucrose density gradient centrifugation and preparative, non-denaturing PAGE
Superdex 200 gel-filtration
-
wild-type and recombinant enzyme
-