1.13.11.57: gallate dioxygenase

This is an abbreviated version, for detailed information about gallate dioxygenase, go to the full flat file.

Reaction

gallate
+
O2
=
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate

Synonyms

DesB, GA dioxygenase, GALA

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.57 gallate dioxygenase

Substrates Products

Substrates Products on EC 1.13.11.57 - gallate dioxygenase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4,5-trihydroxybenzoate + O2
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
show the reaction diagram
DesB has strict substrate specificity for 3,4,5-trihydroxybenzoate (gallate). Biochemical and structural study show the substrate recognition and catalytic mechanisms of extradiol dioxygenase DesB. Mutational analysis reveals that His124 and His192 in the active site are essential to the catalytic reaction of the extradiol dioxygenase DesB. His124, which interacts with OH(4) of the bound gallate, seems to contribute to proper positioning of the substrate in the active site. His192, which is located close to OH (3) of the gallate, is likely to serve as the catalytic base. Glu377' interacts with OH(5) of the gallate and seems to play a critical role in the substrate specificity
-
-
?
gallate + O2
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
show the reaction diagram
gallate + O2
4-oxalomesaconate
show the reaction diagram
pyrogallol + O2
?
show the reaction diagram
additional information
?
-