1.13.11.58: linoleate 9S-lipoxygenase
This is an abbreviated version!
For detailed information about linoleate 9S-lipoxygenase, go to the full flat file.
Word Map on EC 1.13.11.58
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1.13.11.58
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linoleic
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oxylipins
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9-loxs
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lipoxygenases
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9s-hydroperoxide
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13-lipoxygenase
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divinyl
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colneleic
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trihydroxy
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alpha-ketols
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epoxyalcohols
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12r-lox
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9s-hydroperoxy-10e,12z-octadecadienoic
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synthesis
- 1.13.11.58
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linoleic
- oxylipins
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9-loxs
- lipoxygenases
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9s-hydroperoxide
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13-lipoxygenase
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divinyl
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colneleic
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trihydroxy
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alpha-ketols
- epoxyalcohols
- 12r-lox
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9s-hydroperoxy-10e,12z-octadecadienoic
- synthesis
Reaction
Synonyms
15-LOX, 9-lipoxygenase, 9-LO, 9-LOX, 9LOX, 9S-lipoxygenase, arachidonate 9S-LOX, AtLOX-1, AtLOX-5, AtLOX1, AtLOX5, CaLOX1, eLOX3, epidermal lipoxygenase-3, GhLOX1, L-2, linoleate 9-lipoxygenase, linoleate 9S-lipoxygenase, linoleate 9S-lipoxygenase 1, linoleate 9S-lipoxygenase 2, linoleate 9S-lipoxygenase 3, linoleate oxygen oxidoreductase, lipoxygenase, LOX, LOX1, LOX1.1, LOX1.3, LOX12, LOX13, LOX14, LOX1:Md:1c, LOX1c, LOX2, LOX3, LOX4, LOX5, LOX6, LOX7, LOX8, LOX9, LoxA, Nb-9-LOX, Osr9-LOX1, plant LOX-1, POTLX-1, r9-LOX1, seed linoleate 9S-lipoxygenase-3, StLOX1, tuberization protein, type-1 9-LOX, ZmLOX3, ZmLOX5
ECTree
Advanced search results
Engineering
Engineering on EC 1.13.11.58 - linoleate 9S-lipoxygenase
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A562G
the product of the wild-type enzyme is 98.8% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 1.2% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (99.4%). The mutant A562G forms (11S)-hydroperoxyanandamide and 15R-hydroperoxyanandamide in the ratio 3:2
A451G
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the mutant reacts well with arachidonic acid even in the absence of fatty acid hydroperoxides
G567A
site-directed mutagenesis, the mutant converts the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation
A564G
the product of the wild-type enzyme is 99.1% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 0.9% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (71%), plus 16% (5S)-hydroperoxyanandamide. The mutant enzyme A564G forms two additional prominent products, 15-hydroperoxyanandamide (34%) and 9-hydroperoxyanandamide (19%)
I395F
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the regioselectivity of the variant is changed from the n-12 position on C20 PUFA to the n-6 position to form 15S-HFAs
V569W
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the variant has a significantly lower secondx1eoxygenating activity compared to wild type enzyme
additional information
in transgenic lines, L-2, expression is dramatically downregulated, compared to the nontransgenic controls, enzyme knockout in Oryza sativa by hpRNAi expression, transfection via Agrobacterium tumefaciens strain EHA105
additional information
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in transgenic lines, L-2, expression is dramatically downregulated, compared to the nontransgenic controls, enzyme knockout in Oryza sativa by hpRNAi expression, transfection via Agrobacterium tumefaciens strain EHA105
additional information
antisense expression of Osr9-lox1 (asr9lox1), by Agrobacterium tumefaciens-mediated transformation, decrease the amount of wound-induced (Z)-3-hexenal but increase levels of striped stem borer (SSB)-induced linolenic acid, lasmonic acid, salicylic acid and trypsin protease inhibitors
additional information
enzyme knockout in Oryza sativa by hpRNAi expression, transfection via Agrobacterium tumefaciens strain EHA105, in transgenic lines, r9-LOX1 expression is dramatically downregulated, compared to the nontransgenic controls. r9-LOX1 RNAi transgenic lines show 74.33% decrease in nonanal content (formed during oxidation of linoleic acid by lipoxygenase), but a 388.24% increase in acetic acid and 184.84% hexanal (direct products of 13-LOX)
additional information
expression of antisense coding sequence of a specific tuber LOX, the suppression mutant POTLX-1 exhibits a significant reduction in LOX activity in stolons and tubers