1.13.11.6: 3-hydroxyanthranilate 3,4-dioxygenase

This is an abbreviated version, for detailed information about 3-hydroxyanthranilate 3,4-dioxygenase, go to the full flat file.

Reaction

3-Hydroxyanthranilate
+
O2
=
2-amino-3-carboxymuconate semialdehyde

Synonyms

3-HAD, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid oxidase, 3-hydroxyanthranilic acid oxygenase, 3-hydroxyanthranilic oxygenase, 3HAO, EC 1.13.1.6, HAD, oxygenase, 3-hydroxyanthranilate 3,4-di-

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.6 3-hydroxyanthranilate 3,4-dioxygenase

Metals Ions

Metals Ions on EC 1.13.11.6 - 3-hydroxyanthranilate 3,4-dioxygenase

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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
-
the most effective salt, the optimal concentration is 0.035 M
Fe
-
each monomer contains two iron binding sites. The catalytic iron is buried deep inside the beta-barrel with His51, Glu57, and His95 serving as ligands. The other iron site forms an FeS4 center close to the solvent surface in which the sulfur atoms are provided by Cys125, Cys128, Cys162, and Cys165. The two iron sites are separated by 24 A
Fe3+
-
does not seem to bind to the enzyme
HCl
-
during purification of enzyme treatment with acid was used
MgCl2
-
-
NaCl
-
-
Ni2+
-
two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site
additional information
-
comparison of the active sites of Fe(III)-h3HAO and Zn(II)-h3HAO enzymes, overview