220.127.116.11: 3-hydroxyanthranilate 3,4-dioxygenase
This is an abbreviated version!
For detailed information about 3-hydroxyanthranilate 3,4-dioxygenase, go to the full flat file.
3-HAD, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid oxidase, 3-hydroxyanthranilic acid oxygenase, 3-hydroxyanthranilic oxygenase, 3HAO, EC 18.104.22.168, HAD, oxygenase, 3-hydroxyanthranilate 3,4-di-
Molecular Weight on EC 22.214.171.124 - 3-hydroxyanthranilate 3,4-dioxygenase
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predicted from cDNA sequence
PAGE in nondenaturing conditions, pI 4.98 enzyme
PAGE in nondenaturing conditions, pI 5.6 enzyme
ion spray MS, recombinant enzyme
immunoblot analysis, recombinant enzyme
33000 - 34000
gel filtration, readily aggregates to form inactive higher molecular weight oligomers
35000 - 40000
gel filtration, SDS-PAGE
37000 - 38000
gel filtration, SDS-PAGE, sucrose density gradient centrifugation
SDS-PAGE, for the two active enzyme solutions obtained from hydroxyapatite column
SDS-PAGE, nondenaturating PAGE resolves two components, purified by FPLC on protein PakGlass DEAE-4 PW column, one component is N-terminally truncated annexin IV