1.13.11.6: 3-hydroxyanthranilate 3,4-dioxygenase

This is an abbreviated version, for detailed information about 3-hydroxyanthranilate 3,4-dioxygenase, go to the full flat file.

Reaction

3-Hydroxyanthranilate
+
O2
=
2-amino-3-carboxymuconate semialdehyde

Synonyms

3-HAD, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid oxidase, 3-hydroxyanthranilic acid oxygenase, 3-hydroxyanthranilic oxygenase, 3HAO, EC 1.13.1.6, HAD, oxygenase, 3-hydroxyanthranilate 3,4-di-

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.6 3-hydroxyanthranilate 3,4-dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.6 - 3-hydroxyanthranilate 3,4-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, in 0.01 M Tris buffer, pH 7.0, 4 days, no loss of activity
-
-80°C, as homogenate stable for 2 months
-
-90°C, frozen in dry ice-ethanol bath, partially purified enzyme is stable for at least 1 month, purified enzyme is unstable
-
0°C, 0.01 M collidine chloride, 0.01 M potassium chloride, pH 6.5, about 15% loss of activity after 1 month, purified enzyme
-
0°C, 66.7 mM Tris-HCl buffer, pH 8.0, 90 min, approximately 25% loss of activity
-
25°C, 66.7 mM Tris-HCl buffer, pH 8.0, 90 min, approximately 70% loss of activity
-
4°C, overnight, 75% loss of activity
-
4°C, overnight, about 75% loss of activity
-