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1.13.11.73: methylphosphonate synthase

This is an abbreviated version!
For detailed information about methylphosphonate synthase, go to the full flat file.

Word Map on EC 1.13.11.73

Reaction

2-hydroxyethylphosphonate
+
O2
=
methylphosphonate
 +
HCO3-

Synonyms

methylphosphonate synthase, mpnS

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.73 methylphosphonate synthase

Engineering

Engineering on EC 1.13.11.73 - methylphosphonate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q152D
the mutant shows weakened activity compared to the wild type enzyme
Q152H
the mutant shows weakened activity compared to the wild type enzyme
E176H
-
site-directed mutagenesis, the HEPD, EC 1.13.11.72, mutant is bifunctional exhibiting the activity of both 2-hydroxyethylphosphonate dioxygenase (HEPD) and methylphosphonate synthase (MPnS). The product distribution of the mutant is sensitive to a substrate isotope effect, consistent with an isotope-sensitive branching mechanism involving a common intermediate. The introduced histidine does not coordinate the active site metal, unlike the iron-binding glutamate it replaces. More HEPD activity is observed when the reaction is carried out with (R)-2-[2-2H1]-hydroxyethylphosphonate