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1.13.11.84: crocetin dialdehyde synthase

This is an abbreviated version!
For detailed information about crocetin dialdehyde synthase, go to the full flat file.

Word Map on EC 1.13.11.84

Reaction

3beta-hydroxy-8'-apo-beta-carotenal
+
O2
=
crocetin dialdehyde
 +
3beta-hydroxy-beta-cyclocitral

Synonyms

beta-carotene 7',8'oxygenase, carotenoid cleavage dioxygenase 2, CCD2, CCD4.1, CCD4.3, EC 1.14.99.42, ZCD, zeaxanthin 7, 8 (7, 8)-cleavage dioxygenase, zeaxanthin 7,8(7',8')-cleavage dioxygenase, zeaxanthin 7,8-cleavage dioxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.84 crocetin dialdehyde synthase

Cloned

Cloned on EC 1.13.11.84 - crocetin dialdehyde synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CsZCD cDNA in frame with a polyhistidine affinity tag of the pBAD/TOPO ThioFusion vector is expressed in Escherichia coli JM109 cells
expressed in Escherichia coli and in Zea mays endosperm
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Top10 cells
-
expressed in Nicotiana tabacum
-
expressed in transgenic Micro-Tom tomato (Solanum lycopersicum)
-
gene CsZCD, quantitative RT-PCR expression analysis