1.13.11.85: exo-cleaving rubber dioxygenase
This is an abbreviated version!
For detailed information about exo-cleaving rubber dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.85
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1.13.11.85
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xanthomonas
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latex
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c-type
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polycis-1,4-isoprene
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oxygenases
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dihaem
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rubber-degrading
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gordonia
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steroidobacter
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latex-clearing
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waste
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peroxidases
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analysis
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synthesis
- 1.13.11.85
- xanthomonas
- latex
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c-type
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polycis-1,4-isoprene
- oxygenases
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dihaem
-
rubber-degrading
- gordonia
-
steroidobacter
-
latex-clearing
- waste
- peroxidases
- analysis
- synthesis
Reaction
+ n O2 = n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
Synonyms
heme-dependent rubber oxygenase, LATA, poly(cis-1,4-isoprene)-cleavage enzyme, roxA, RoxB, RubA, rubber oxygenase, rubber oxygenase A
ECTree
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General Information
General Information on EC 1.13.11.85 - exo-cleaving rubber dioxygenase
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malfunction
metabolism
physiological function
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Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
malfunction
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Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
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the enzyme is excreted by Xanthomonas to functionalize and cleave the inert latex biopolymer poly(cis-1,4-isoprene) into the more soluble (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal units
metabolism
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the enzyme is essential for the poly(cis-1,4-isoprene) rubber utilization of its host strain
metabolism
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the enzyme is essential for the poly(cis-1,4-isoprene) rubber utilization of its host strain
metabolism
-
the enzyme is essential for the poly(cis-1,4-isoprene) rubber utilization of its host strain
metabolism
-
the enzyme is essential for the poly(cis-1,4-isoprene) rubber utilization of its host strain
metabolism
-
the enzyme is essential for the poly(cis-1,4-isoprene) rubber utilization of its host strain
metabolism
-
the enzyme is essential for the poly(cis-1,4-isoprene) rubber utilization of its host strain
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expression of gene functionally complements a Xanthomonas sp. 35Y RoxA deletion mutant
physiological function
expression of gene functionally complements a Xanthomonas sp. 35Y RoxA deletion mutant
physiological function
expression of gene functionally complements a Xanthomonas sp. 35Y RoxA deletion mutant
physiological function
inactivation of the chromosomal roxA gene results in inability of Xanthomonas sp. to produce active RoxA and to utilize rubber as a sole source of carbon and energy
physiological function
RoxA is isolated with O2 stably bound to the active site heme iron. Activation and cleavage of O2 require binding of polyisoprene, and the substrate needs to use hydrophobic access channels to reach the deeply buried active site of RoxA. Both residues H312 and Y462 are ideally positioned to form hydrogen bonds that arrest the substrate chain, so that the bond connecting the third and fourth monomer is in close proximity to the O2 ligand at heme 1. After oxidative cleavage, the resulting 2-oxo-4,8-dimethyl-trideca-4,8-diene-1-al product dissociates, and RoxA slides along the isoprene chain until the terminus is again arrested by hydrogen bonds