1.13.11.87: endo-cleaving rubber dioxygenase
This is an abbreviated version!
For detailed information about endo-cleaving rubber dioxygenase, go to the full flat file.
Reaction
Cleavage of cis-1,4-polyisoprene polymers into a mixture of compounds, including a C20 compound ((4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal), a C25 compound ((4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal), a C30 compound ((4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal), and larger isoprenologes such as C35, C40, C45, and higher analogues
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Synonyms
GPOL_174p00150, GPOL_c48310, latex clearing protein, latex-clearing protein, Lcp, Lcp protein, Lcp1, lcp1VH2, Lcp2, lcp2VH2, LcpK30, polyisoprene-cleaving rubber oxygenase, roxA, RoxB, rubber oxidizing protein, rubber oxygenase, rubber oxygenase B
ECTree
Inhibitors
Inhibitors on EC 1.13.11.87 - endo-cleaving rubber dioxygenase
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1,10-phenanthroline
1 mM, 20% inhibition
2,2'-bipyridyl
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15% residual activity at 10 mM
alpha-tocopherol
1 mM, 40% inhibition
ascorbate
1 mM, 20% inhibition
carotene
1 mM, 40% inhibition
CO
oxygen consumption by RoxB is inhibited when carbon monoxide is added to an ongoing polyisoprene cleavage reaction
Diethylthiocarbamate
1 mM, 56% inhibition
disodium 4,5-dihydroxy-1,3-benzenedisulfonate
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45% residual activity at 10 mM
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Dithionite
1 mM, 15% inhibition
dithiothreitol
10 mM, 90% inhibition
ethanol
5%, 10% inhibition
ethyl acetate
the presence of ethyl acetate does not completely inhibit the activity of the enzyme
ethyl xanthogenate
1 mM, 7% inhibition
ethylxanthate
-
46% residual activity at 10 mM
pentane
the presence of pentane does not completely inhibit the activity of the enzyme
squalene
1 mM, 10% inhibition
Diethyl dithiocarbamate
82 % inhibition. No effect on the UV(vis) spectrum of the enzyme and this excludes a direct effect of the inhibitor at the heme site
Diethyl dithiocarbamate
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almost complete inhibition (99%) at 1 mM
Diethyl dithiocarbamate
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diethyldithiocarbamate
1 mM, 50% inhibition
diethyldithiocarbamate
1 mM, 56% inhibition
EDTA
-
25% residual activity at 10 mM
EDTA
1 mM, 30% inhibition
imidazole
-
about 50% residual activity at 1 mM
imidazole
inhibits by 35% and 85% at 1 mM and 10 mM
imidazole
1 mM, 48% inhibition
imidazole
binding of this compounds to the active heme site inhibits the enzyme
Phenanthroline
-
19% residual activity at 10 mM
Phenanthroline
1 mM, 10% inhibition
Pyridine
-
about 70% residual activity at 1 mM
Pyridine
binding of this compounds to the active heme site inhibits the enzyme
SDS
0.1%, complete inhibition
additional information
carbon monoxide has no inhibitory effect on the polyisoprene cleavage
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additional information
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carbon monoxide has no inhibitory effect on the polyisoprene cleavage
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additional information
not inhibitory: EDTA up to 10 mM, carbon monoxide
-
additional information
the use of 1 or 10 mM EDTA has no effect on the rubber-cleaving activity of RoxB
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