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1.13.11.88: isoeugenol monooxygenase

This is an abbreviated version!
For detailed information about isoeugenol monooxygenase, go to the full flat file.

Word Map on EC 1.13.11.88

Reaction

isoeugenol
+
O2
=
Vanillin
 +
acetaldehyde

Synonyms

iem, Iem720, ISO, NCED

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.88 isoeugenol monooxygenase

Temperature Stability

Temperature Stability on EC 1.13.11.88 - isoeugenol monooxygenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 20
conversion after 12 h increases from 67.0 to 84.1% with the temperature increase from 15 to 20°C, and it decreases beyond 20°C
20
stable for 30 min
20 - 40
at 20°C and 30°C, more than 90% of initial activity residue is detected after 150 min incubation. However, its activity is decreased when incubated at temperatures higher than 40°C, about 50% of its initial activity is detected at 40°C after 30 min of incubation and almost inactivated at 50°C
25 - 35
-
the wild type enzyme shows 53.2% residual activity after 15 min at 35°C. The enzyme has half-lives of 967.2, 49.8 and 7.8 min at 25, 30, and 35°C, respectively
30
30 min, 7% loss of activity
50
30 min, complete loss of activity