1.13.11.9: 2,5-dihydroxypyridine 5,6-dioxygenase

This is an abbreviated version!
For detailed information about 2,5-dihydroxypyridine 5,6-dioxygenase, go to the full flat file.

Reaction

Synonyms

2,5-DHP dioxygenase, 2,5-dihydroxypyridine dioxygenase, 2,5-dihydroxypyridine oxygenase, 2,5DHP dioxygenase, EC 1.13.1.9, NicX, NicX protein, oxygenase, 2,5-dihydroxypyridine 5,6-di-, pyridine-2,5-diol dioxygenase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.9 2,5-dihydroxypyridine 5,6-dioxygenase

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Results	in table
3	Activating Compound
278	AA Sequence
2	Application
1	CAS Registry Number
4	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
3	General Information
7	General Stability
24	Inhibitors
2	KM Value [mM]
2	Localization
10	Metals/Ions
7	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
136	Organism
1	Oxidation Stability
7	Pathway
18	PDB ID
5	pH Optimum
2	Protein Variants
5	Purification (Commentary)
1	Reaction
3	Reaction Type
14	Reference
2	Source Tissue
5	Specific Activity [micromol/min/mg]
5	Storage Stability
28	Substrates and Products (Substrate)
8	Subunits
23	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Stability [°C]

Cloned

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Cloned on EC 1.13.11.9 - 2,5-dihydroxypyridine 5,6-dioxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

functional overexpression in Escherichia coli strain BL21 (DE3)

Pseudomonas putida

-

710759

PCR-amplification of nicX gene, overexpression of nix gene in Escherichia coli BL21(DE3) cells with plasmid pETNicX, for knockout mutants transformation of pKnicX plasmid into Pseudomonas putida KT2440 as recipient, using Escherichia coli DH10B(pKnicX) as donor strain, Escherichia coli HB101 (pRK600) as helper strain

Pseudomonas putida

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700940

phylogenetic analysis

Cupriavidus campinensis

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742294

the nicX gene is overexpressed in Escherichia coli BL21(DE3) cells containing plasmid pETNicX

Pseudomonas putida

-

700940