1.13.12.13: Oplophorus-luciferin 2-monooxygenase

This is an abbreviated version!
For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.13

Reaction

Oplophorus luciferin
+
O2
=
oxidized Oplophorus luciferin
+
CO2
+
hnu

Synonyms

19kOLase, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.13 Oplophorus-luciferin 2-monooxygenase

General Information

General Information on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase

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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
Q9GV45; Q9GV46
the enzyme is a member of the coelenterazine-utilizing luciferases such as Renilla luciferase and Gaussia luciferase
malfunction
Q9GV45; Q9GV46
recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway
physiological function
Q9GV45; Q9GV46
the secreted luciferase of the deep-sea shrimp Oplophorus gracilirostris catalyzes the oxidation of coelenterazine to emit blue light (lambda_max = 460 nm)
additional information
Q9GV45; Q9GV46
the coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure of the catalytic subunit KAZ