1.13.12.13: Oplophorus-luciferin 2-monooxygenase

This is an abbreviated version!
For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.13

Reaction

Oplophorus luciferin
+
O2
=
oxidized Oplophorus luciferin
+
CO2
+
hnu

Synonyms

19kOLase, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.13 Oplophorus-luciferin 2-monooxygenase

Purification

Purification on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
form inclusion bodies of recombinant Escherichia coli, solubilization with 6 M urea
-
recombinant His-tagged catalytic subunit mutant nanoKAZ from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis, wild-typeKAZ is purified from inclusion bodies using 6 M urea-nickel chelate affinity column chromatography
Q9GV45; Q9GV46
recombinant His-tagged catalytic wild-type and mutant enzymes and catalytic subunits from Escherichia coli by nickel affinity chromatgraphy
Q9GV45; Q9GV46
using Ni-NTA chromatography
-
using nickel-chelate affinity column
-