1.13.12.16: nitronate monooxygenase

This is an abbreviated version!
For detailed information about nitronate monooxygenase, go to the full flat file.

Reaction

Synonyms

2-nitropropane dioxygenase, EC 1.13.11.32, NAO, ncd2, nitroalkane oxidase, nitroalkane-oxidizing enzyme, nitronate monooxygenase, nitropropane dioxygenase, NMO, NMO-Nc, NMO-Ws, nmoA, Npd1, Npd2, Npd3, Npd4, Npd5, Npd6, oxidase, nitroalkane, oxygenase, 2-nitropropane di-, P3N monooxygenase, Pa-NMO, PA4202, protein PA4202, Rv1894c, Sa-NAO

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.16 nitronate monooxygenase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
3	Activating Compound
3508	AA Sequence
2	CAS Registry Number
13	Cloned(Commentary)
32	Cofactor
5	Crystallization (Commentary)
3	Expression
8	General Information
3	General Stability
84	Inhibitors
57	kcat/KM [mM/s]
20	Ki Value [mM]
122	KM Value [mM]
9	Metals/Ions
16	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
57	Organism
3	Pathway
16	PDB ID
26	pH Optimum
5	pH Range
1	pH Stability
11	Protein Variants
21	Purification (Commentary)
11	Reaction
3	Reaction Type
40	Reference
4	Source Tissue
9	Specific Activity [micromol/min/mg]
2	Storage Stability
234	Substrates and Products (Substrate)
14	Subunits
76	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
3	Temperature Range [°C]
6	Temperature Stability [°C]
67	Turnover Number [1/s]

Purification

print visible entries

print all entries

Purification on EC 1.13.12.16 - nitronate monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

5 entries

DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography

Neurospora crassa

Q01284

656289

DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography; recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography

Neurospora crassa

Q01284

656289

HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography

Pseudomonas aeruginosa

Q9I4V0

674590

HisTrap column chromatography

Metarhizium brunneum

A0A6C0WVH0, A0A6C0WVK2, A0A6C0WW14, A0A6C0WWH2

763994

phenyl Sepharose column chromatography and DEAE Sepharose column chromatography

Neurospora crassa

-

764031

purified to homogeneity

Cyberlindnera mrakii

-

639224

recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography

Neurospora crassa

Q01284

656289

recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3)pLysS by affinity chromatography

Pseudomonas aeruginosa

Q9HWH9

745306

to homogeneity

4 entries

using Ni-NTA chromatography

Mycobacterium tuberculosis

-

728077

-

Neurospora crassa

-

639232, 672094, 685283

-

Fusarium oxysporum

-

639225

-

Cyberlindnera mrakii

Q12723

639222

-

Cyberlindnera mrakii

-

639227, 639235

-

Cyberlindnera mrakii

Q12723

684712

to homogeneity

Neurospora crassa

-

639232

to homogeneity

Fusarium oxysporum

-

639225

to homogeneity

Cyberlindnera mrakii

-

639223, 639222

to homogeneity

Cyberlindnera mrakii

Q12723

639222