1.13.12.16: nitronate monooxygenase

This is an abbreviated version!
For detailed information about nitronate monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.16

Reaction

ethylnitronate
+
O2
=
acetaldehyde
+
nitrite
+
other products

Synonyms

2-nitropropane dioxygenase, NAO, nitroalkane oxidase, nitroalkane-oxidizing enzyme, nitronate monooxygenase, nitropropane dioxygenase, NMO, NMO-Nc, NMO-Ws, nmoA, oxidase, nitroalkane, oxygenase, 2-nitropropane di-, P3N monooxygenase, Pa-NMO, PA4202, protein PA4202, Rv1894c, Sa-NAO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.16 nitronate monooxygenase

Purification

Purification on EC 1.13.12.16 - nitronate monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography
DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography; recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography
HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography
purified to homogeneity
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography
recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3)pLysS by affinity chromatography
to homogeneity
using Ni-NTA chromatography
-