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1.13.12.19: 2-oxoglutarate dioxygenase (ethene-forming)

This is an abbreviated version!
For detailed information about 2-oxoglutarate dioxygenase (ethene-forming), go to the full flat file.

Word Map on EC 1.13.12.19

Reaction

2-oxoglutarate
+
O2
=
Ethene
 + 3 CO2 +
H2O

Synonyms

2-oxoglutarate-Fe(II) oxygenase, 2OG-Fe(II) oxygenase, EFE, ethylene forming enzyme, ethylene-forming enzyme, More, PsEFE

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.19 2-oxoglutarate dioxygenase (ethene-forming)

Cloned

Cloned on EC 1.13.12.19 - 2-oxoglutarate dioxygenase (ethene-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons with Pseudomonas digitatum and Pseudomonas chrysogenum, recombinant expression in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisae. Different cultivation factors on ethylene formation in Saccharomyces cerevisiae expressing the EFE in continuous cultures are investigated. Main finding is that oxygen availability is crucial for ethylene production. By employing three different nitrogen sources it is shown that the nitrogen source available can both improve and impair the ethylene productivity. N-Source/yield ethylene (microgram/g glucose): (NH4)2SO4/164, glutamate/233, glutamate+arginine/96.8
-
expression in Escherichia coli
expression in Nicotiana tabacum
gene efe, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree
gene efe, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, heterologous expression of the single efe gene from Pseudomonas syringae results in ethylene production in a number of hosts including Escherichia coli, Saccharomyces cerevisiae, Pseudomonas putida, Trichoderma viride, Trichoderma reesei, tobacco, and cyanobacteria. Methods overview
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
gene efe, recombinant enzyme expression in Synechocystis sp. strain PCC 6803, stable ethylene production through the integration of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5'-AGGAGG-3) as the ribosome-binding site, at the slr0168 neutral site. Increase in ethylene production is achieved twofold by RBS screening, improvement of ethylene production from a single gene copy of efe, using multiple tandem promoters and by putting our best construct on an RSF1010-based broad-hostself-replicating plasmid, which has a higher copy number than the genome
gene efe, recombinant expression in Escherichia coli strain MG1655, importance of promoter strength on the expression of enzyme EFE in Escherichia coli with stronger promoters producing elevated levels of ethylene, e.g. the Amaranthus hybridus chloroplast psbA promoter (PpsbA), expression method optimization, overview
-
gene efe, recombinant expression of N-terminally His-tagged or N-terminally His-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3)
His-tagged form of enzyme is generated in Escherichia coli BL21 Gold (DE3) cells
-
recombinant expression of wild-type and mutant enzymes