1.13.12.19: 2-oxoglutarate dioxygenase (ethene-forming)

This is an abbreviated version!
For detailed information about 2-oxoglutarate dioxygenase (ethene-forming), go to the full flat file.

Reaction

Synonyms

2-oxoglutarate-Fe(II) oxygenase, 2OG-Fe(II) oxygenase, EFE, ethylene forming enzyme, ethylene-forming enzyme, More, PsEFE

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.19 2-oxoglutarate dioxygenase (ethene-forming)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
6	Activating Compound
2077	AA Sequence
6	Application
16	Cloned(Commentary)
2	Crystallization (Commentary)
21	General Information
11	Inhibitors
9	kcat/KM [mM/s]
10	KM Value [mM]
14	Metals/Ions
6	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
30	Organism
3	Pathway
27	PDB ID
3	pH Optimum
1	pH Range
1	pI Value
73	Protein Variants
4	Purification (Commentary)
9	Reaction
24	Reference
1	Specific Activity [micromol/min/mg]
37	Substrates and Products (Substrate)
6	Subunits
51	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
9	Turnover Number [1/s]

Crystallization

print visible entries

print all entries

Crystallization on EC 1.13.12.19 - 2-oxoglutarate dioxygenase (ethene-forming)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

analysis of crystal structure of the enzyme in complex with several ligands, detailed overview

Pseudomonas savastanoi pv. phaseolicola

P32021

742750

purified recombinant enzyme in three structures: of enzyme in complex with manganese and 2-oxoglutarate, of enzyme in complex with with manganese and bis-Tris-propane buffer, and enzyme in complex with iron, L-Arg, and N-oxalylglycine, hanging drop vapour diffusion method, mixing of 0.003 ml of 12-20 mg/ml protein solution with 0.003 ml of reservoir solution containing 17.5-25% w/v PEG 3350 and PEG 6000, 0.1 M bis-Tris-propane, pH 7.0, 0.04 M sodium/potassium phosphate, 3-5 mM MnCl2, and with or without 10 mM 2-oxoglutarate, and equilibration against 0.5 ml reservoir solution, at 20°, microseeding, method optimization, X-ray diffraction structure determination and analysis at 1.08-1.55 A resolution, single-wavelength anomalous diffraction data obtained from selenomethionine-derivatized PsEFE:Mn:bis-Tris-propane crystals, structure modeling

Pseudomonas savastanoi pv. phaseolicola

P32021

743692