1.13.12.19: 2-oxoglutarate dioxygenase (ethene-forming)

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For detailed information about 2-oxoglutarate dioxygenase (ethene-forming), go to the full flat file.

Reaction

Synonyms

2-oxoglutarate-Fe(II) oxygenase, 2OG-Fe(II) oxygenase, EFE, ethylene forming enzyme, ethylene-forming enzyme, More, PsEFE

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.19 2-oxoglutarate dioxygenase (ethene-forming)

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Results	in table
6	Activating Compound
2077	AA Sequence
6	Application
16	Cloned(Commentary)
2	Crystallization (Commentary)
21	General Information
11	Inhibitors
9	kcat/KM [mM/s]
10	KM Value [mM]
14	Metals/Ions
6	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
30	Organism
3	Pathway
27	PDB ID
3	pH Optimum
1	pH Range
1	pI Value
73	Protein Variants
4	Purification (Commentary)
9	Reaction
24	Reference
1	Specific Activity [micromol/min/mg]
37	Substrates and Products (Substrate)
6	Subunits
51	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
9	Turnover Number [1/s]

Purification

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Purification on EC 1.13.12.19 - 2-oxoglutarate dioxygenase (ethene-forming)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

from cell-free extract

Pseudomonas syringae

P32021

715784

recombinant enzyme

Pseudomonas syringae

P32021

715773

recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by affinityy chromatography, desalting gel filtration, tag cleavage by human SenP2 protease, followed by another step of affinityy chromatography, and gel filtration

Pseudomonas savastanoi pv. phaseolicola

P32021

743692

recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through

Pseudomonas savastanoi pv. phaseolicola

P32021

741936