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1.13.12.2: lysine 2-monooxygenase

This is an abbreviated version!
For detailed information about lysine 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.2

Reaction

L-lysine
+
O2
=
5-Aminopentanamide
 +
CO2
 +
H2O

Synonyms

davB, L-AAO/MOG, L-amino acid oxidase/monooxygenase, L-LOX/MOG, L-lysine 2-monooxygenase, L-lysine monooxygenase, L-lysine oxidase/monooxygenase, L-lysine-2-monooxygenase, lysine monooxygenase, lysine oxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.2 lysine 2-monooxygenase

Cloned

Cloned on EC 1.13.12.2 - lysine 2-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2-monooxygenase (DavB) and delta-aminovaleramidase (DavA) are coexpressed in Escherichia coli BL21(DE3) to produce nylon-5 monomer 5-aminovalerate from L-lysine
gene davB, recombinant expression in Corynebacterium glutamicum strain LYS-12, coexpression with Pseudomonas putida gene davA, encoding 5-aminovaleramidase
-
gene davB, recombinant expression in Escherichia coli strain WL3110, coexpression with gene davA, encoding 5-aminovaleramidase
-
gene davB, recombinant expression in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase, lysine specific permease LysP, and PP2911, a 4-aminobutyrate transporter, since Escherichia coli is unable to assimilate L-lysine and to secrete 5-aminovalerate
gene davB, recombinant expression in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase, recombinant expression of gene davB as N-terminally His-tagged enzyme in Corynebacterium glutamicum, a highly L-lysine producing strain BE using the sod promoter and the transcription factor Tu, EC 3.6.5.3
-
gene davB, recombinant expression of codon-optimized enzyme DavB in Escherichia coli strains BL21(DE3) and BL-22A-RB-YB, coexpression with gene davA, encoding 5-aminovaleramide amidohydrolase
gene davB, recombinant expression of His-tagged enzyme DavB in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase
gene laao/mog, recombinant expression of His-tagged wild-type enzyme L-amino acid oxidase/monooxygenase in Escherichia coli strain BL21(DE3), selenomethionine-labeled enzyme is expressed in Escherichia coli strain BL21 CodonPlus (DE3)-RIL-X
recombinantly expressed in Escherichia coli. Wild-type Escherichia coli enzyme can not convert lysine to 5-aminovalerate, whereas recombinant Escherichia coli enzyme expressing the davBA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase produces 5-aminovalerate from lysine with a 64% conversion yield
-