1.13.12.24: calcium-regulated photoprotein

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For detailed information about calcium-regulated photoprotein, go to the full flat file.

Reaction

Synonyms

aequorin, alcium-activated photoprotein, berovin, BFP-aq, blue fluorescent protein, Ca2+-binding photoprotein, Ca2+-regulated photoprotein, clytin, halistaurin, mitrocomin, mnemiopsin, mnemiopsin 1, mnemiopsin 2, mnemiopsin1, obelin, phialidin

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.24 calcium-regulated photoprotein

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Results	in table
4	Activating Compound
10	AA Sequence
7	Application
26	Cloned(Commentary)
6	Crystallization (Commentary)
3	General Information
2	General Stability
11	Inhibitors
20	Metals/Ions
6	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
41	Organism
5	pH Optimum
1	pH Stability
57	Protein Variants
16	Purification (Commentary)
5	Reaction
41	Reference
1	Source Tissue
28	Substrates and Products (Substrate)
6	Subunits
30	Synonyms
1	Systematic Name
13	Temperature Stability [°C]

Systematic Name

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Systematic Name on EC 1.13.12.24 - calcium-regulated photoprotein

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SYSTEMATIC NAME

IUBMB Comments

coelenterazine:oxygen 2-oxidoreductase (decarboxylating, calcium-dependent)

Ca2+-regulated photoproteins are found in a variety of bioluminescent marine organisms, mostly coelenterates, and are responsible for their light emission. The best studied enzyme is from the jellyfish Aequorea victoria. The enzyme tightly binds the imidazolopyrazinone derivative coelenterazine, which is then peroxidized by oxygen. The hydroperoxide is stably bound until three Ca2+ ions bind to the protein, inducing a structural change that results in the formation of a 1,2-dioxetan-3-one ring, followed by decarboxylation and generation of a protein-bound coelenteramide in an excited state. The calcium-bound protein-product complex is known as a blue fluorescent protein. In vivo the energy is transferred to a green fluorescent protein (GFP) by Forster resonance energy transfer. In vitro, in the absence of GFP, coelenteramide emits a photon of blue light while returning to its ground state.