220.127.116.11: tryptophan 2-monooxygenase
This is an abbreviated version, for detailed information about tryptophan 2-monooxygenase, go to the full flat file.
IaaM, oxygenase, tryptophan 2-mono-, Pgt-IaaM, PGTG_11658, TMO, tryptophan 2-monooxygenase, tryptophan monooxygenase
1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
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Cloned on EC 18.104.22.168 - tryptophan 2-monooxygenase
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evidence for extrachromosomal nature of gene involved in indole-3-acetic acid synthesis
expression in Escherichia coli
gene IaaM, the gene is constitutively expressed in plant host Arabidopsis thaliana via Ti plasmid-encoded T-DNA insertion in tranfected cells, and no transcription factor further activates its promoter IGR1b, that contains cis-regulatory binding elements. Genetic organization, overview. Transcription factor ARR1 can regulate transcription of the IaaH or IaaM genes. Promoter IGR1 drives expression of IaaH and IaaM and contains only one Wbox and AuxRE sequence motif, and this is more closely localized upstream of the IaaM than that of the IaaH TATA box
gene PGTG_11658 or Pgt-IaaM, transgenic expression of gene Pgt-IaaM in Arabidopsis thaliana haustoria causes a typical auxin expression phenotype and promotes susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, transgenic expression of gene Pgt-IaaM in Triticum aestivum leaves, phenotypes, overview. Silencing of Pgt-IaaM by wheat host-induced gene silencing reduces the pathogenicity of Puccinia graminis. Expression of Pgt-IaaM in Arabidopsis thaliana displays pleiotropic auxin-related phenotypes