1.13.12.3: tryptophan 2-monooxygenase

This is an abbreviated version!
For detailed information about tryptophan 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.3

Reaction

L-tryptophan
+
O2
=
(indol-3-yl)acetamide
+
CO2
+
H2O

Synonyms

IaaM, oxygenase, tryptophan 2-mono-, Pgt-IaaM, PGTG_11658, TMO, tryptophan 2-monooxygenase, tryptophan monooxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.3 tryptophan 2-monooxygenase

Engineering

Engineering on EC 1.13.12.3 - tryptophan 2-monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C339A
-
slight decrease in activity with tryptophan or methionine
C511S
-
high decrease in activity with tryptophan or methionine
H338N
-
slight decrease in activity with tryptophan or methionine
K365M
mutation decreases the kcat and kcat/Km (L-tryptophan) values by 60000- and 2 millionfold compared to wild-type, respectively
R98A
-
decrease in Vmax, increase in Km-value of tryptophan
R98K
-
decrease in Vmax, increase in Km-value of tryptophan
W466F
mutation decreases the kcat value less than 2fold and the kcat/Km (L-tryptophan) value only 5fold compared to wild-type
W466M
mutation results in an enzyme lacking flavin and detectable activity
Y413A
-
200fold reduction in first-order rate constant for reduction, 33fold decrease in rate constant for oxidation, conversion of enzyme to oxidase
Y413F
-
35fold reduction in first-order rate constant for reduction, 11fold decrease in rate constant for oxidation, conversion of enzyme to oxidase