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analysis
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analysis of luciferase inhibitors by quantitative high-throughput screening and comparison of and structure-activity relationship using various luciferase-based detection reagents
analysis
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development of a bioluminiscent probe composed of peptide EYFP and luciferase for near-real-time single-cell imaging using bioluminescence resonance energy transfer BRET. The probe exhibits enhanced luciferase luminescence intensity and appropriate subcellular distribution when it is fused to targeting-signal peptides or histone H2AX. It allows high spatial and temporal resolution microscopy of living cells
analysis
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the use of control Renilla luciferase vectors as normalizers requires that they not be influenced by any variables in the experiment. The zinc finger transcription factor WT1 effect on luciferase activation varies from no significant effect in 293 and PC3 cells to strong enhancement in LNCaP cells treated with the androgen analog R1881. Hormone enhances WT1-mediated activation of luciferase and these interactions require an intact WT1 zinc finger DNA binding domain
analysis
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for analysis of the Hsp90 chaperone activity in complex with cochaperone Cdc37, split Renilla luciferase protein fragment-assisted complementation bioluminescence can be utilized to study the full-length human Hsp90-Cdc37 complex and to identity critical residues and their contributions for Hsp90/Cdc37 interaction in living cells
analysis
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Renilla luciferase is used in assay development for measurement of mitochondrial fusion, quantification via split-Renilla luciferase complementation in HeLa cells, validation of the Renilla luciferase reporter system for mitochondrial fusion, overview
analysis
an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells
analysis
establishment and evaluation of an indirect autophagy flux assay based on monitoring the degradation of an autophagosome-associated fusion protein Rluc-LC3 by luminescence detection. The Rluc-LC3 assay is useful for the identification of genes, miRNAs, and small molecules that regulate autophagy flux in mammalian cells
analysis
Renilla luciferase is a bioluminescent enzyme which is broadly used as a reporter protein in molecularbiosensors
analysis
enhanced-sensitivity, synthetically facile reporter fusion to merge the bioluminescence output of Gaussia luciferase with the biotin-binding ability of tamavidin 2. The fusion construct enables direct bacterial expression of a reporter system incorporating two functionalities in a 1:1 stoichiometric relationship. Using a cold-shock expression system, highly concentrated construct can be obtained from standard culture volumes while retaining essentially native protein activity. The fusion construct can be included in a standard target-bridged assay design for the sensitive detection of miRNA targets
analysis
luciferase can be functionally expressed in Staphylococcus aureus and can be used as a biosensor for the agr quorum sensing system which employs autoinducing peptides to control virulence. Luciferase is linked to the P3 promoter of the agr operon and biosensor strains are validated by evaluation of chemical agent-mediated activation and inhibition of agr. Use of Luciferase enables quantitative assessment of agr activity
biotechnology
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expression of native gene and commercial synthetic gene, optimized for expression, in several cell lines and in mouse. Use of synthetic gene as primary reporter gene with high sensitivity in living rodents
biotechnology
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use of enzyme as a reporter is dependent on the promotor driving its expression, the presence of co-transfected transgenes, and the androgen responsiveness of the cell line used
biotechnology
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use of native coelenterazine and its derivatives e, -f, -h, as substrates for use in cell culture and living animals
biotechnology
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popular reporter enzyme for gene expression and biosensor applications
biotechnology
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split luciferase complementation is applied to study dynamic protein-protein interactions in live bacteria. Nonspecific inhibition of Rluc activity by small molecule effectors compromises the utility of this technique in measuring dynamic protein-protein interactions
biotechnology
an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells
diagnostics
Renilla Luciferase (RLuc) is a blue light emitter protein which can be applied as a valuable tool in medical diagnosis
diagnostics
the split Renilla luciferase complementation assay (SRLCA) is one of the techniques that detect protein-protein interactions. The SRLCA is based on the complementation of the LN and LC non-functional halves of Renilla luciferase fused to possibly interacting proteins which after interaction form a functional enzyme and emit luminescence. The SRLCA can specifically detect the BGLF4/Hsp90 interaction and provide a reference to develop inhibitors that disrupt the Epstein-Barr virus kinase BGLF4 and heat shock protein Hsp90 interaction
diagnostics
the enzyme is a good research tool as a reporter protein and bioimaging probes, yielding blue light using the substrate coelenterazine. However the applications are limited since RLuc is unstable under various conditions
molecular biology
Renilla sp.
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development of a reverse genetic model to characterize the pathway of replication and pathogenesis of the SARS coronavirus. Renilla luciferase is used as a reporter gene and inserted into the backbone of the infectious clone of SARS coronavirus to replace ORF 7a/b (SARS wt-Luc), which is believed to have apoptotic effects on host cells. Recombinant viruses with luciferase constructs are isolated and shown to stably maintain the Renilla luciferase gene and to express subgenomic mRNA encoding luciferase. SARS wt-Luc is a viable virus that allows studies of the effect of subgenomic manipulation on virus efficiacy, both in replication and subgenomic production. This approach offers an alternative to plaque assay analysis in testing the efficiency of anti-SARS agents
molecular biology
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dual luciferase enzyme assay system for reporter gene analysis combining both the firefly luciferase enzyme and the Renilla luciferase enzyme in a nonproprietary buffer
molecular biology
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modification of the photoprotein aequorin by attaching selected fluorophores at a unique site on the protein. This will allow for in vitro transfer of bioluminescent energy from aequorin to the fluorophore thus creating an artificial jellyfish. The fluorophores are selected such that the excitation spectrum of the fluorophore overlaps with the emission spectrum of aequorin. By modifying aequorin with different fluorophores, bioluminescent labels with different emission maxima are produced, which will allow for the simultaneous detection of multiple analytes
molecular biology
Renilla sp.
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Renilla luciferase, fused to biospecific sequences such as engineered antibodies, can be administered systemically to provide a novel, sensitive method for optical imaging based on expression of cell surface receptors in living organism
molecular biology
Renilla sp.
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the development of variants of Renilla luciferase, which exhibit significantly improved properties compared with the native enzyme, will allow enhanced sensitivity in existing luciferase-based assays as well as enable the development of novel probes labeled with the luciferase protein
molecular biology
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when aequorin is microinjected into cleavage-stage zebrafish embryos, it is largely used up by about 24 hours. Thus, it is not possible to image Ca2+ signals from later stages of zebrafish development using this approach. Transient expression of apoaequorin (i.e., the protein component of aequorin) using aeq-mRNA in zebrafish embryos and then reconstitution of intact aequorin in vivo by loading the coelenterazine cofactor into the embryos separately provides a valuable tool for monitoring Ca2+ signaling during the 2448 h post fertilization period of zebrafish development. Thus, it effectively extends the aequorin-based Ca2+-imaging window by an additional 24 hours
molecular biology
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sensitive reproter for studies of gene expression, promoter activity, protein-protein interactions, signal transduction, tumor cell growth, response to therapy
molecular biology
an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells
molecular biology
Renilla luciferase (Rluc) from Renilla reniformis is an appropriate protein reporter for the detection of specific molecular targets due to its bioluminescent feature, although its relatively low stability limits the application
pharmacology
Renilla sp.
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investigations into regulation and functional roles of kinases
pharmacology
Renilla sp.
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used as an assay for assessing potential liver toxicity by measuring GADD45-beta induction as an control for increased DNA damage
additional information
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detection of tumor cells bearing a particular surface marker
additional information
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Gluc reporter as a sensitive marker in elucidation endoplasmic reticulum stress in mammalian cells
additional information
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imaging of T cells in the context of a competent immune system, bioluminescent imaging
additional information
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monitor processing of proteins through the secretory pathway and endoplasmic reticulum
additional information
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visualize proteins being secreted by exocytosis