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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
+
ATP
=
firefly oxyluciferin
+
CO2
+
AMP
+
diphosphate
+
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

KM Value

KM Value on EC 1.13.12.7 - firefly luciferase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.176
(4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 8.5, temperature not specified in the publication
0.07
(4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 8.5, temperature not specified in the publication
0.061
(4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 9.1, temperature not specified in the publication
0.02
(4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 8.5, temperature not specified in the publication
0.00025 - 0.0008
5,5-dimethyl-luciferyl-O-adenosine monophosphate
0.00015 - 140
ATP
0.00076 - 25
D-firefly luciferin
0.001 - 2.5
D-luciferin
0.00038 - 0.0126
D-luciferyl-O-adenosine monophosphate
0.0149
dehydroluciferyl-adenylate
-
-
0.0982
L-luciferin
-
-
0.00741 - 0.0163
lauric acid
0.0136
linoleic acid
-
0.0136
linolenic acid
-
-
0.0072 - 0.218
Luciferin
0.0006 - 0.0127
luciferyl-O-adenosine monophosphate
0.025 - 6.2
MgATP2-
0.0147
oxyluciferin
-
-
additional information
additional information
-