1.13.12.7: firefly luciferase

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Word Map on EC 1.13.12.7

1.13.12.7

bioluminescence

luminescence

chemiluminescence

emit

renilla

noninvasive

luciferases

cypridina

photon

lentiviral

adenovirus

click

luciferase-expressing

herpes

engraft

nude

co-transfected

cytomegalovirus

camera

electroporation

simplex

hsp70

emitter

replication-deficient

excited-state

promoter-driven

charge-coupled

nonviral

nanoluc

non-invasively

gaussia

luminol

intramyocardial

coelenterazine

bicistronic

yellow-green

molecular biology

light-emitting

cap-independent

aequorin

biotechnology

red-shifted

dnaj

luminometer

grpe

polyethylenimine

bioimaging

luciferase-based

medicine

photoproteins

glow

analysis

multicolor

brighter

Reaction

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

1.13.12.7 firefly luciferase

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Results	in table
30	Activating Compound
817	AA Sequence
24	Application
1	CAS Registry Number
80	Cloned(Commentary)
11	Cofactor
4	Crystallization (Commentary)
11	General Information
22	General Stability
212	IC50 Value
348	Inhibitors
7	kcat/KM [mM/s]
37	Ki Value [mM]
338	KM Value [mM]
8	Localization
43	Metals/Ions
31	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
186	Organism
2	Pathway
33	PDB ID
76	pH Optimum
17	pH Range
2	pH Stability
6	pI Value
185	Protein Variants
56	Purification (Commentary)
1	Reaction
11	Reaction Type
181	Reference
2	Renatured (Commentary)
23	Source Tissue
25	Specific Activity [micromol/min/mg]
7	Storage Stability
222	Substrates and Products (Substrate)
16	Subunits
164	Synonyms
1	Systematic Name
52	Temperature Optimum [°C]
5	Temperature Range [°C]
56	Temperature Stability [°C]
52	Turnover Number [1/s]

Purification

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Purification on EC 1.13.12.7 - firefly luciferase

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11 entries

a more efficient method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE) is designed. Downstream processing of luciferase from Photinus pyralis is carried out using polymer/salt aqueous two phase system (ATPS) at 4°C. The enzyme is observed to preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification (13.69 fold) and enzyme activity recovery (118.34%) are observed in the system containing 4.0% (w/w) PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21