1.14.11.11: hyoscyamine (6S)-dioxygenase

This is an abbreviated version!
For detailed information about hyoscyamine (6S)-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.11

Reaction

L-hyoscyamine
+
2-oxoglutarate
+
O2
=
(6S)-hydroxyhyoscyamine
+
succinate
+
CO2

Synonyms

6H6, AaH6H, AtH6H, H6H, HnH6H, hyoscyamine 6-hydroxylase, hyoscyamine 6beta-hydroxylase, hyoscyamine-6beta-hydroxylase, oxygenase, hyoscyamine 6beta-di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.11 hyoscyamine (6S)-dioxygenase

Cloned

Cloned on EC 1.14.11.11 - hyoscyamine (6S)-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA from Hyoscyamus niger is simultaneously introduced into Nicotiana tabacum using particle bombardment and expressed under the control of the CaMV 35S promoter
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DmH6H gene, expression of the His-tagged enzyme in Escherichia coli
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DNA and amino acid sequence determination and analysis, overexpression of N- or C-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3), AbH6H loses of the first amino acid methionine during transcription
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DNA and amino acid sequence determination and analysis, overexpression of N-terminally His- or GST-tagged enzyme in Escherichia coli strain BL21 (DE3)
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expressed in Atropa baetica
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expressed in Duboisia leichhardtii
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expressed in Nicotiana tabacum and Hyoscyamus muticus
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expression in Escherichia coli
expression in Escherichia coli as a fusion protein to maltose-binding protein
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expression in Escherichia coli XL1B
expression in Saccharomyces cerevisiae in tagged and untagged form
expression of wild-type and mutant enzymes in Escherichia coli
gene h6h, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, gene expression profiling and real-time RT-PCR analysis
overexpression of the enzyme in hairy roots of transgenic Anisodus acutangulus using Agrobacterium tumefaciens strain C58C1 transfection method, coexpression with tropinone reductase I
T1 progeny of transgenic Atropa belladonna, in which putrescine N-methyltransferase (EC 2.1.1.53) from Nicotiana tabacum (NtPMT) and hyoscyamine 6beta-hydroxylase (EC 1.14.11.14) from Hyoscyamus niger (HnH6H) are overexpressed, are established to investigate tropane alkaloids biosynthesis and distribution in ripe fruits, leaves, stems, primary roots and secondary roots under field conditions. Both NtPMT and HnH6H are detected at the transcriptional level in transgenic plants, whereas they are not detected in wild-type plants. Metabolically engineered Atropa belladonna based on overexpressing NtPMT and HnH6H shows a chemotype of rich scopolamine that greatly improves the commercial and pharmaceutical properties of Atropa belladonna
the gene encoding hyosyamine 6-hydroxylase is placed under the regulation of the CaMV 35S promoter and introduced into the genome of a scopolamine-rich Duboisia hybrid by a binary vector system using Agrobacterium rhizogenes strain LBA9402. The scopolamine levels in the resulting engineered hairy root lines increases up to three times compared to wild-type hairy roots, but there is no clear increase in the engineered regenerated plants
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the h6hcDNA is amplified from Brugmansia candida total RNA preparations, it is inserted in frame to the trx tag into the pET32a(+) vector. Escherichia coli Origami strains are used as host for the expression. Produce of high quantities of a soluble and functional enzyme
transformation through Agrobacterium rhizogenes infection
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