1.14.11.15: gibberellin 3beta-dioxygenase

This is an abbreviated version!
For detailed information about gibberellin 3beta-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.15

1.14.11.15

20-oxidase

ga1

dellas

phytochrome

ga-deficient

paclobutrazol

ent-kaurene

atga20ox1

ga-dependent

ent-copalyl

ga2ox2

ent-kaurenoic

ga-mediated

Reaction

Synonyms

(giberrellin-20),2-oxoglutarate: oxygen oxidoreductase (3beta-hydroxylating), AtGA3ox1, CYP115, DWARF1 D1 protein, DWF1, GA 3-oxidase, GA 3-oxidase 2, GA 3beta-hydroxylase, GA 3ox, GA3 oxidase, GA3 oxidase 1, GA3-oxidase, GA3-oxidase 1, GA3ox, GA3ox1, GA3OX4, gibberellin 3 oxidase, gibberellin 3 oxidase 1, gibberellin 3-beta-dioxygenase 1, gibberellin 3-beta-dioxygenase 2-1, gibberellin 3-beta-dioxygenase 2-2, gibberellin 3-beta-dioxygenase 2-3, gibberellin 3-oxidase, gibberellin 3-oxidase 1, gibberellin 3-oxidase 2, gibberellin 3beta dioxygenase, gibberellin 3beta-hydroxylase, MtGA3ox1, OsGA3ox1, oxygenase, gibberellin 3beta-di-, SbGA3ox2, SSL, TfGA3ox1, ZmGA3ox2

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

1.14.11.15 gibberellin 3beta-dioxygenase

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Results	in table
6	Activating Compound
120	AA Sequence
1	CAS Registry Number
16	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
2	Expression
40	General Information
48	Inhibitors
9	KM Value [mM]
4	Localization
6	Metals/Ions
3	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
75	Organism
4	Pathway
1	PDB ID
4	pH Optimum
2	pH Range
1	pH Stability
4	Protein Variants
9	Purification (Commentary)
1	Reaction
3	Reaction Type
64	Reference
38	Source Tissue
4	Specific Activity [micromol/min/mg]
111	Substrates and Products (Substrate)
2	Subunits
176	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Purification

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Purification on EC 1.14.11.15 - gibberellin 3beta-dioxygenase

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Oryza sativa Japonica Group

Q6AT12

764523

C18-reverse HPLC column chromatography

Rumex palustris

676607

high performance liquid chromatography

Triticum aestivum

Q3I409, Q3I410, Q3I411

676701

immobilised metal affinity chromatography and Superdex 200 gel filtration

partial

recombinant maltose-binding protein-fused enzyme from Escherichia coli by amylose affinity chromatography

Arabidopsis thaliana

Q39103

725686

rosette leaves are frozen in liquid nitrogen and ground to a fine powder, protein extracted with 50 mM Tris-HCl, pH 8.0, containing 200 mM NaCl, 5 mM EDTA, 0.1% Tween 20 and a proteinase inhibitor mixture, SDS-PAGE

Arabidopsis thaliana

700861

transgenic plants are ground in liquid nitrogen, total cellular extracts suspended in 1X SDS-PAGE sample loading buffer

Arabidopsis thaliana

700749