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1.14.11.2: procollagen-proline 4-dioxygenase

This is an abbreviated version!
For detailed information about procollagen-proline 4-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.2

Reaction

Procollagen L-proline
+
2-oxoglutarate
 +
O2
=
procollagen trans-4-hydroxy-L-proline
 +
succinate
 +
CO2

Synonyms

A085R, alpha (I) subunit, anthrax-P4H, AT-P4H-1, At-P4H-2, BaP4H, C-P4H, C-P4H alpha subunit (III), C-P4H alpha(I), C-P4H alpha(III), CePHY-1/PHY-2/PDI2, collagen proline hydroxylase, collagen prolyl 4-hydroxylase, collagen prolyl 4-hydroxylase (type-II), collagen prolyl 4-hydroxylase 1, collagen prolyl-4-hydroxylase, collagen prolyl-4-hydroxylase alpha subunit 2, CP4H, CP4H1, CrP4H-1, DmP4H, egg-laying abnormal-9 prolyl hydroxylase, Egl nine homolog, EGLN, EGLN prolyl hydroxylase, EGLN3, EGLN3 hydroxylase, GBAA_4459, HIF prolyl hydroxylase, HIF prolyl-4-hydroxylase, HIF-1alpha-specific prolyl-hydroxylase, HIF-P4H-1, HIF-P4H-2, HIF-P4H-3, HPH, HPH-2, HuPH4-I, HuPH4-II, hydroxylase, collagen proline, hypoxia inducible factor prolyl-4-hydroxylase domain-containing protein, hypoxia inducible factor-prolyl hydroxylase, hypoxia-inducible factor -1alpha-type prolyl 4-hydroxylase, hypoxia-inducible factor prolyl hydroxylase, hypoxia-inducible factor-1alpha-prolyl-hydroxylase 2, More, NtP4H1.1, P4H, P4H alpha1, P4H-1, P4H1, P4ha1, P4ha2, P4Halpha(I), P4Halpha(II), P4Halpha(III), P4Halpha1, PBCV-1 P4H, peptidyl proline hydroxylase, PH, PHD, PHD-1, PHD1, PHD2, PHD3, procollagen prolyl 4-hydroxylase, procollagen-proline dioxygenase, proline 4-hydroxylase, proline hydroxylase, proline protocollagen hydroxylase, proline, 2-oxoglutarate dioxygenase, proline,2-oxoglutarate 4-dioxygenase, prolyl 4-hydroxylase, prolyl 4-hydroxylase A085R, prolyl hydroxylase, prolyl hydroxylase domain 1, prolyl hydroxylase domain containing protein, prolyl hydroxylase domain enzyme, prolyl hydroxylase domain protein 2, prolyl hydroxylase-1, prolyl hydroxylase-3, prolyl-4-hydroxylase, prolyl-4-hydroxylase alpha I, prolyl-4-hydroxylase alpha II, prolyl-4-hydroxylase alpha subunit 2, prolyl-4-hydroxylase alpha1, prolyl-4-hydroxylase-alpha1, prolyl-4-hydroxylases, prolyl-glycyl-peptide, 2-oxoglutarate:oxygen oxidoreductase, 4-hydroxylating, prolyl4-hydroxylase, prolylprotocollagen dioxygenase, prolylprotocollagen hydroxylase, protocollagen hydroxylase, protocollagen proline 4-hydroxylase, protocollagen proline dioxygenase, protocollagen proline hydroxylase, protocollagen prolyl hydroxylase, PSB-II, Skp1 prolyl hydroxylase, type I C-P4H, type I proly 4-hydroxylase, type I proyl 4-hydroxylase, type II proyl 4-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.2 procollagen-proline 4-dioxygenase

Cloned

Cloned on EC 1.14.11.2 - procollagen-proline 4-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a human embryonic kidney (HEK) 293 cell line is generated expressing FLAG-tagged PHD2
-
alpha-subunit
-
beta-subunit
-
catalytic alpha subunit
-
cloning of the alpha subunit of the enzyme, coexpression in insect cells with the Drosophila protein-disulfide isomerase polypeptide produces an active enzyme tetramer, coexpression in insect cells with human protein-disulfide isomerase polypeptide produces also small amounts of a hybrid tetramer
-
cloning of the alpha subunit of the enzyme, expression of the alpha subunit together with human protein disulfide isomerase/beta subunit in insect cells by baculovirus vectors
-
cloning of the alpha-I subunit and of a second mouse alpha-subunit isoform, termed the alpha-II subunit, expression of the alpha-II subunit together with human protein disulfide isomerase/beta subunit in insect cells by baculovirus vectors
-
cloning of the alpha-II subunit, coexpression with the beta subunit in insect cells
co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2, in Sf9 cell
-
co-expression of prolyl-4-hydroxylase with collagen type I and lysyl hydroxylase 3 in transgenic tobacco plants resulting in production of bioactive, post-translationally modified, heterotrimeric, human recombinant type-I collagen, overview
-
determination of PHD3 mRNA by real-time PCR
-
expressed in Escherichia coli
expressed in Escherichia coli strains BL21 and RB791
-
expressed in MCF7, U2OS, HEK-293T, MEF and HUVEC cells
-
expressed in tobacco BY-2 cells as a GFP-fusion protein
-
expressed in Trichoplusia ni and Drosophila melanogaster S2 cells
-
expression by Escherichia coli BL21(DE3)
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3) pLysS cells
expression in Escherichia coli, the genome of virus-1 encodes a 242-amino acid polypeptide that shows a distinct amino acid sequence similarity to the C-terminal half of the catalytic alpha subunit of animal enzymes, the genome contains many open reading frames for proteins with proline-rich repeats
-
expression in insect cell
-
expression in insect cells and HEK-293 cells
expression in Sf9 cells
-
expression in Sf9 insect cells
expression in Sf9 insect cells, HEK293T cells, in vitro transcription/translation in rabbit reticulocyte lysate
expression of histidine and cysteine mutant alpha subunits together with the wild-type beta subunit in insect cells by means of baculovirus vectors
-
expression of PHY-1/PHY-2/(PDI-2)2 P4H tetramer in insect cells
-
expression of selenomethionine-labeled anthrax-P4H in Escherichia coli strain BL21(DE3)
-
expression of the enzyme with a N-terminal His6-SUMO fusion protein in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli strain Origami DE3
-
expression, of alpha (I) subunit in Escherichia coli
-
gene bap4h, recombinant expression in Escherichia coli strain BL21 star DE3 pLysS cells
Q81LZ8
gene GBAA_4459, recombinant enzyme expression in Escherichia coli strain BL21 Star (DE3) pLysS
Q81LZ8
gene P4ha1, quantitative real-time PCR enzyme expression analysis
gene P4HA2, enzyme expression analysis, P4HA2 is associated with expression of Col1A1, Col3A1, and Col4A1 during breast cancer development and progression
gene P4halpha1, quantitative real-time PCR enzyme expression analysis
identification of three cDNAs encoding putative alpha subunits of the enzyme, co-expression of one of these with protein-disulfide isomerase in a catalytically active form in an insect system, genomic structure
in vitro transcription/translation in rabbit reticulocyte lysate
infection of cells with recombinant viruses encoding type XII minicollagen together with various combinations of recombinant viruses encoding the wild-type or mutant enzyme, analysis of the contributions of the various cellular post-translational modifications to the assembly of trimeric type XII minicollagen, study of the conditions required for the production of disulfide-bonded type XII minicollagen in insect cells using the baculovirus expression system
-
preparation of cDNA clones for the two subunits, expression in Escherichia coli
-
recombinant expression of the three isozymes
-
recombinant human PHD2 (corresponding to residues 178-426 of the full-length PHD2 sequence) is expressed as a GST-fusion protein in Escherichia coli
-
the genome encodes six enzyme-like polypeptides, that show an identity of 21-27% to the catalitically important C-terminal regions of the human enzyme alpha-I and alpha-II subunits. Cloning of one of the genes, recombinant enzyme expressed in insect cells and in Escherichia coli