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1.14.11.27: [histone H3]-dimethyl-L-lysine36 demethylase

This is an abbreviated version!
For detailed information about [histone H3]-dimethyl-L-lysine36 demethylase, go to the full flat file.

Word Map on EC 1.14.11.27

Reaction

a [histone H3]-N6,N6-dimethyl-L-lysine36
+ 2 2-oxoglutarate + 2 O2 =
a [histone H3]-L-lysine36
 + 2 succinate + 2 formaldehyde + 2 CO2

Synonyms

dKDM2, dKDM4A, dRAF, GASC1, H3-K36 demethylase, H3-K36-specific demethylase, H3K36 demethylase, H3K36me2 demethylase, H3K36me2 histone demethylase, H3K36me2-specific demethylase, histone H3 lysine 36 demethylase, histone H3 lysine 36 dimethylspecific demethylase, histone-lysine(H3-K36) demethylase, Jhd1, JHDM1A, Jhdm1b/Kdm2b, JmjC domain histone demethylase, JmjC domain-containing histone demethylase 1, JmjC domain-containing histone demethylase 1A, JmjC domain-containing histone demethylation protein 1, JMJD2, JMJD2A, JMJD5, JMJD7, Kdm2a, KDM2B, KDM2b/JHDM1b, KDM4, KDM4A, KDM4B, Kdm4c, KDM4D, KDM8, lysine demethylase, More, Ndy1, PfJmjC1, PfSET1, scJHDM1, SET1, [histone-H3]-lysine-36 demethylase 1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.27 [histone H3]-dimethyl-L-lysine36 demethylase

Cloned

Cloned on EC 1.14.11.27 - [histone H3]-dimethyl-L-lysine36 demethylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, chromosomal location and association analysis , the porcine JHDM1A gene encodes 1,162 amino acids and contains JmjC, F-box, and CXXC zinc-finger domains, which coding sequence and deduced protein shares 91 and 99% similarity with human JHDM1A, respectively
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ectopic expression of either wild-type Jhdm1a or H212A point mutant in the liver of diabetic ob/ob mice
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ectopic expression of Jhdm1a in HeLa and wild-type and enzyme-deficient Hep-G2 cells via lentivirus transfection
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expression analysis by quantitative reverse transcriptase PCR, overexpression of wild-type Jhdm1b in HeLa and HEK293 cells
expression in mouse embryo fibroblast
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expression of Flag-tagged protein
expression of N-terminally His-tagged KDM4A in Escherichia coli
expression of N-terminally His-tagged KDM4B in Escherichia coli
expression of N-terminally His-tagged KDM4C in Escherichia coli
gene Jmjd5, FLAG-His6 tagged mutant H319A is cloned into the pDON-5 Neo plasmid to produce retroviruses, shRNA-expressing retroviruses are used for silencing of the enzyme, expression of C-terminally His-tagged JMJD5 in Escherichia coli, quantitative reverse transcription PCR expression analysis
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gene KDM4A, recombinant expression of EGFP-tagged full-length and truncated enzymes versions
gene KDM4B, recombinant isozyme expression in U2OS-TetON stable cell line that conditionally expresses the fusion protein EGFP-KDM4B
gene KDM4C, recombinant isozyme expression in U2OS-TetON stable cell line that conditionally expresses the fusion protein EGFP-KDM4C, recombinant expression of EGFP-tagged full-length and truncated, andmutant enzymes versions
gene KDM4D is Y chromosome encoded and a truncated enzyme variant compared to KDM4A-C
gene MAL8P1.111, located on chromosome 8, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, overview
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gene PFF1440w, located on chromosome 6, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, overview
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recombinant Ndy1 overexpression in mouse embryonic fibroblasts, quantitative real-time reverse transcriptase PCR expression analysis
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