1.14.11.29: hypoxia-inducible factor-proline dioxygenase

This is an abbreviated version!
For detailed information about hypoxia-inducible factor-proline dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.29

Reaction

hypoxia-inducible factor-L-proline
+
2-oxoglutarate
+
O2
=
hypoxia-inducible factor-trans-4-hydroxy-L-proline
+
succinate
+
CO2

Synonyms

EGLN, EGLN1, Egln2, EGLN3, factor inhibiting HIF, FIH, HIF hydroxylase, HIF prolyl, HIF prolyl 4-hydroxylase, HIF prolyl hydroxylase, HIF-1alpha PHD3, HIF-1alpha prolyl hydroxylase 3, HIF-alpha prolyl-hydroxylase, HIF-P4H-1, HIF-P4H-2, HIF-P4H-3, HIF-PH, Hif-prolyl hydroxylase, HIF-prolyl hydroxylase domain 2, HIF-prolyl hydroxylase-2, HPH-1, HPH-2, HPH-3, hydroxylase domain enzyme, hypoxia-inducible factor prolyl hydroxylase 2, hypoxia-inducible factor prolyl hydroxylase domain 2, PHD, PHD1, PHD2, PHD3, proline hydroxylase domain 2, prolyl 4-hydroxylase, prolyl hydroxylase, prolyl hydroxylase domain, prolyl hydroxylase domain protein, prolyl hydroxylase domain protein 2, prolyl hydroxylase-2, prolyl-4-hydroxylase 2, prolyl-4-hydroxylase domain 2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.29 hypoxia-inducible factor-proline dioxygenase

Cloned

Cloned on EC 1.14.11.29 - hypoxia-inducible factor-proline dioxygenase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
expression in Spodoptera frugiperda Sf9 cells and Escherichia coli; expression in Spodoptera frugiperda Sf9 cells and Escherichia coli; expression in Spodoptera frugiperda Sf9 cells and Escherichia coli
expression of His-tagged human PHD3 in Escherichia coli strain BL21(DE3)
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expression of N-terminally GST-tagged PHD2 residues 178-426 in Escherichia coli strain L21(DE3)
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expression of N-terminally MBP-tagged PHD3 in Escherichia coli strain BL21(DE3)pRR692; expression of PHD1-(1-407) in Spodoptera frugiperda Sf9 cells using the baculovirus expression vector, expression of His- and Strep-tagged tobacco etch virus-PHD1 in Escherichia coli strain BL21(DE3)pRR692; expression of PHD2-(1-426) in Spodoptera frugiperda Sf9 cells uing the baculovirus expression vector, expression of His- and Strep-tagged tobacco etch virus-PHD2 in Escherichia coli strain BL21(DE3)pRR692
expression of PHD2 catalytic domain residues 177-426, expression of N-terminally GST-tagged PHD2 in Escherichia coli strain BL21(DE3)
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gene EGLN1
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gene EGLN1, recombinant expression in HEK 293T cells from vector pRL-TK containing Renilla reniformis luciferase gene, real-time RT-PCR enzyme expression analysis
recombinant expression of GST-tagged PHD2
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the coding region of human PHD3 DNA is optimized by using synonymous codons according to the code bias of Escherichia coli, expression of soluble and active N-terminally Trx- and C-terminally His-tagged human PHD3 in Escherichia coli strain BL21(DE3)pLysS at lower induction temperature of 25C
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