1.14.11.30: hypoxia-inducible factor-asparagine dioxygenase

This is an abbreviated version!
For detailed information about hypoxia-inducible factor-asparagine dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.30

Reaction

hypoxia-inducible factor-L-asparagine
+
2-oxoglutarate
+
O2
=
hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine
+
succinate
+
CO2

Synonyms

asparaginyl hydroxylase, asparaginyl-hydroxylase, factor inhibiting HIF, factor inhibiting HIF-1, factor inhibiting hypoxia inducible factor-1alpha, factor-inhibiting HIF, factor-inhibiting hypoxia-inducible factor, FIH, FIH hydroxylase, HIF asparagine hydroxylase, HIF asparaginyl hydroxylase, HIF hydroxylase, hypoxia-inducible factor asparagine hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.30 hypoxia-inducible factor-asparagine dioxygenase

Purification

Purification on EC 1.14.11.30 - hypoxia-inducible factor-asparagine dioxygenase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
recombinant dox-inducible FLAG-tagged FIH from U2-OS cells by immunoaffinity chromatography
-
recombinant GST-tagged PHD2 by glutathiione affinity chromatography
-
recombinant His- and MBP-tagged FIH from Escherichia coli strain BL21(DE3)pRR692 by nickel affinity and anion exchange chromatography
-