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1.14.11.33: DNA oxidative demethylase

This is an abbreviated version!
For detailed information about DNA oxidative demethylase, go to the full flat file.

Word Map on EC 1.14.11.33

Reaction

DNA-base-CH3
+
2-oxoglutarate
+
O2
=
DNA-base
+
formaldehyde
+
succinate
+
CO2

Synonyms

1-methyladenine-DNA dioxygenase, ABH1, ABH2, ABH3, AlkB, AlkB homolog 1, ALKBH2, ALKBH3, ALKBH8, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, FTO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.33 DNA oxidative demethylase

Purification

Purification on EC 1.14.11.33 - DNA oxidative demethylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-cellulose column chromatography, S-Sepharose column chromatography, Ni-NTA column chromatography, and Mono-S cation exchange column chromatography
DEAE-cellulose column chromatography, SP Sepharose cation exchange column chromatography, and Mono-S cation exchange column chromatography
-
GSTrap column chromatography
-
His-tagged AlkB is purified by Ni-NTA agarose column chromatography, untagged Alkb is purified by DEAE-cellulose column chromatography, Sepharose column chromatography, and Mono S column chromatography
His-tagged enzyme is purified by Ni-NTA agarose column chromatography
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Mono-Q column chromatography
Ni-NTA column chromatography and SP-Sepharose cation exchange affinity column chromatography
-
Ni-NTA column chromatography and UnoS column chromatography
TALON metal affinity resin column chromatography
Talon metal affinity resincolumn chromatography