1.14.11.4: procollagen-lysine 5-dioxygenase

This is an abbreviated version!
For detailed information about procollagen-lysine 5-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.4

Reaction

[procollagen]-L-lysine
+
2-oxoglutarate
+
O2
=
[procollagen]-(2S,5R)-5-hydroxy-L-lysine
+
succinate
+
CO2

Synonyms

2-oxoglutarate 5-dioxygenase 2, collagen lysine hydroxylase, Jmjd6, L230, LH, LH1, LH2, LH2 (long), LH2a, LH2b, LH3, lysine hydroxylase, lysine, 2-oxoglutarate 5-dioxygenase, lysine-2-oxoglutarate dioxygenase, lysyl 5S-hydroxylase, lysyl hydroxylase, lysyl hydroxylase 1, lysyl hydroxylase 2, lysyl hydroxylase 2 (long), lysyl hydroxylase 2a, lysyl hydroxylase 2b, lysyl hydroxylase 3, Lysyl hydroxylase-2b, lysyl-hydroxylase 1, lysylhydroxylase 2, lysylprotocollagen dioxygenase, More, oxygenase, protocollagen lysine, di-, peptidyl-lysine, 2-oxoglutarate: oxygen oxidoreductase, peptidyllysine, 2-oxoglutarate:oxygen 5-oxidoreductase, PLOD, Plod1, PLOD2, Plod2a, Plod2b, PLOD2iso1, PLOD2iso2, PLOD3, procollagen lysyl hydroxylase 2, procollagen-lysine, procollagen-lysine 2-oxoglutarate 5-dioxygenase, procollagen-lysine, 2-oxoglutarate 5-dioxygenase, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2, protocollagen lysine hydroxylase, protocollagen lysyl hydroxylase, telopeptide lysyl hydroxylase, TLH

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.4 procollagen-lysine 5-dioxygenase

Cloned

Cloned on EC 1.14.11.4 - procollagen-lysine 5-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
all 3 isozymes LH1-3, LH2 in 2 splicing variants, are expressed in as His-tagged proteins in Spodoptera frugiperda Sf9 cells via baculovirus infection system with the signal peptide at the N-terminus in isozymes LH2a and b and LH3 being exchanged for the signal peptide of isozyme LH1
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cloning of 3 isozymes LH1-3, with 2 splicing variants of isozyme LH2, LH2a and LH2b, stable and functional expression in CHO-K1 cells
DNA and amino acid sequence determination and analysis of mutant DNA from an Ehlers-Danlos syndrome type IVA patient, expression of mutant enzyme W446G in an insect cell system via baculovirus infection
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endogenous LH2 alternate splicing pattern and conservation analysis of LH2 genomic sequence., overview. Construction and expression of a functional LH2 minigene in HEK-293 cells, human neonatal skin fibroblasts, and mouse embryonic fibroblasts. The TIA proteins play a role in the regulation of the alternative splicing of LH2, overview
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expressed in H5 insect cells
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expression in Escherichia coli, isoform 3 gene product possesses the collagen glycosyltransferase activity, but not isoform 1 and 2
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expression in insect cells using a baculovirus vector
expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, and functional expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3)
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expression in various human tissues
expression of 40 amino acid residues of the C-terminal end of isozyme LH1 fused to human cathepsin D and c-Myc-tagged and 2 mutant variants thereof in COS-7 cells
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expression of a synthetic gene encoding LH3, SEQ ID, in Nicotiana tabacum leaves. Co-expression of the human genes encoding recombinant heterotrimeric collagen type I, rhCOL1, the human prolyl-4-hydroxylase, and the lysyl hydroxylase 3, all responsible for key posttranslational modifications of collagen. Plants coexpressing all five vacuole-targeted proteins generate intact procollagen yields ofabout 2% of the extracted total soluble protein, overview
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expression of active LH2b and of antisense construct in MC3T3-E1 cells, the latter suppresses the endogenous enzyme
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expression of GFP-tagged Jmjd6 in HEK-293 cells, GFP pulldown interaction analysis with U2AF65, overview
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expression of His-tagged full-length JMJD6 and JMJD61-343 in Escherichia coli strain Rosetta
expression of wild-type and mutantenzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
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gene dPlod or CG6199, maps to cytological position 68B1 on the left arm of the third chromosome, DNA and amino acid sequence determination and analysis, sequence comparisons
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gene encoding isozyme LH1, DNA and amino acid sequence determination and analysis, quantitative expression analysis
gene encoding isozyme LH2a, DNA and amino acid sequence determination and analysis, quantitative expression analysis
gene encoding isozyme LH3, DNA and amino acid sequence determination and analysis, quantitative expression analysis
gene Plod1, quantitative RT-PCR enzyme expression analysis
gene Plod1, real-time RT-PCR enzyme expression analysis
gene PLOD2
gene PLOD2, binding of the TGFbeta1 pathway related transcription factors SMAD3 and SP1-mediated TGFbeta1 enhanced PLOD2 expression and might be correlated to an increase of acetylated histone H3 and H4 at the PLOD2 promoter. Depletion of SMAD3 reduces gene PLOD2 acetylated H3 and H4, histone methylation marks at the PLOD2 promoter depicted an increase of the active histone mark H3K79me2, a decrease of the repressive H4K20me3 mark, but no role for the generally strong transcription-related modifications: H3K4me3, H3K9me3 and H3K27me3. TGFbeta1 induces a SP1- and SMAD3-dependent recruitment of histone modifying enzymes to the PLOD2 promoter other than the currently known TGFbeta1 downstream co-activators and epigenetic modifications. Quantitative real-time PCR enzyme expression analysis
gene PLOD2, DNA and amino acid sequence determination and analysis
gene PLOD2, encoding for splice variants LH2a and LH2b, quantitative expression analysis of LH2b
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gene PLOD2, large-scale transfection with N-terminal His8 and human growth hormone (hGH) tags transfection with polyethylenimine and recombinant expression of wild-type enzyme, N-terminally truncated enzyme mutant, and of inactive mutant D689A in engineered CHO cells and secretion to the cell culture medium
gene Plod2, real-time RT-PCR enzyme expression analysis
gene PLOD3, DNA and amino acid sequence determination and analysis, quantitative expression analysis
gene PLOD3, expression in COS-7 and HT-1080 cells, the enzyme is secreted into the culture medium, expression of enzyme mutants in COS-7 cells
gene Plod3, expression of recombinant V5/His-tagged LH3 protein in HEK-293 cells
gene Plod3, quantitative RT-PCR enzyme expression analysis
gene Plod3, real-time RT-PCR enzyme expression analysis
genes PLOD1 and PLOD2, real-time quantitative reverse transcription PCR
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genes Plod2a and Plod2b, quantitative RT-PCR enzyme expression analysis
genomic structure of isozyme LH2, expression analysis of the 2 splicing variants of isozyme LH2
overexpression in HT-1080 cells, expression of fragments in Escherichia coli
quantitative LH gene expression analysis by realtime PCR, genes LH2a and LH2b DNA and amino acid sequence analysis using RT-PCR
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transient transfection of HA-tagged LH1 in 714 mouse embryonic fibroblasts