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1.14.11.45: L-isoleucine 4-hydroxylase

This is an abbreviated version!
For detailed information about L-isoleucine 4-hydroxylase, go to the full flat file.

Word Map on EC 1.14.11.45

Reaction

L-isoleucine
+
2-oxoglutarate
 +
O2
=
(4S)-4-hydroxy-L-isoleucine
 +
succinate
 +
CO2

Synonyms

IDO, isoleucine 4-hydroxylase, L-isoleucine dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.45 L-isoleucine 4-hydroxylase

Engineering

Engineering on EC 1.14.11.45 - L-isoleucine 4-hydroxylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N126H
-
mutant obtained by directed evolution, 2.8fold increase in activity
N126H/T130K
-
catalytic efficiency is 2.4fold and 3.0fold higher than wild-type with Ile and 2-oxoglutaric acid as substrates
T130K
-
mutant obtained by site-specific mutagenesis, 1.7fold increase in activity
I162T
-
specific activity is 1.9fold higher than that of wild-type
I162T/T182N
-
double mutant with improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively
T182N
-
mutant achieves up to 70% conversion of Ile
L27I/E80D/G169H/S182D
catalytic efficiency, thermal stability and catalytic rate are significantly improved compared with those of wild-type IDO
additional information
mutants with improved activity are obtained by a strategy dependent on the restoration of succinate-minus Escherichia coli cell growth by the coupling of L-Ile hydroxylation and the oxidation of 2-oxoglutarate to succinate