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1.14.11.51: DNA N6-methyladenine demethylase

This is an abbreviated version!
For detailed information about DNA N6-methyladenine demethylase, go to the full flat file.

Word Map on EC 1.14.11.51

Reaction

N6-methyladenine in DNA
+
2-oxoglutarate
 +
O2
=
adenine in DNA
 +
formaldehyde
 +
succinate
 +
CO2

Synonyms

6mA DNA demethylase, ALKBH1, DMAD, DNA 6mA demethylase, NMAD, NMAD-1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.51 DNA N6-methyladenine demethylase

Engineering

Engineering on EC 1.14.11.51 - DNA N6-methyladenine demethylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D186A
-
inactive
C118A
m6A demethylase activity similar to wild-type, about 25% decrease in apurinic/apyrimidinic lyase activity
C118A/C129A
about 50% decrease in m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity
C129A
about 90% decrease in m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity
D233A
H113A/C118A/C129A/H134A
no residual m6A demethylase activity, about 55% decrease in apurinic/apyrimidinic lyase activity
H231A
H287A
K133A
about 50% decrease in m6A demethylase activity, about 45% decrease in apurinic/apyrimidinic lyase activity
R338A
residue plays an important role in substrate recognition and catalysis
R344A
mutation in highly conserved residue, strong decrease in demethylation ability
Y184A
mutation in highly conserved residue, strong decrease in demethylation ability
D233A
reduced activity of the recombinant mutant protein
K158A
mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1
R159A
mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1
R160A
mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1
R167A
mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1
R169A
mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1
S235D
mutation disrupts N6-mA demethylation activity
S235E
mutation disrupts N6-mA demethylation activity