1.14.11.55: ectoine hydroxylase
This is an abbreviated version!
For detailed information about ectoine hydroxylase, go to the full flat file.
Word Map on EC 1.14.11.55
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1.14.11.55
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2-oxoglutarate-dependent
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halophil
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5-hydroxyectoine
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halomonas
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salexigens
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ironii
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non-heme-containing
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hydroxylases
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alaskensis
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sphingopyxis
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elongata
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stereo-specific
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osmostress
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mechanosensitive
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tetrahydropyrimidine
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ectabc
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stutzeri
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osmolarity
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virgibacillus
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co-substrate
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halotolerant
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maritimus
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chassis
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salina
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region-selective
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thermoregulated
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nitrosopumilus
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chromohalobacter
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thermoprotection
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alkaliphilic
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hydrothermalis
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sitting-drop
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synthesis
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extremophiles
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prop
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apo-form
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monosodium
- 1.14.11.55
-
2-oxoglutarate-dependent
-
halophil
- 5-hydroxyectoine
-
halomonas
- salexigens
-
ironii
-
non-heme-containing
- hydroxylases
- alaskensis
-
sphingopyxis
- elongata
-
stereo-specific
-
osmostress
-
mechanosensitive
-
tetrahydropyrimidine
-
ectabc
- stutzeri
-
osmolarity
-
virgibacillus
-
co-substrate
-
halotolerant
- maritimus
-
chassis
- salina
-
region-selective
-
thermoregulated
- nitrosopumilus
-
chromohalobacter
-
thermoprotection
-
alkaliphilic
- hydrothermalis
-
sitting-drop
- synthesis
-
extremophiles
-
prop
-
apo-form
-
monosodium
Reaction
Synonyms
Csal_3003, ectD, EctE, ectoine dioxygenase, HELO_4008, REctD, Sala_2952, SCATT_10620, ThpD
ECTree
1.14.11.55 ectoine hydroxylaseAdvanced search results
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Application
Application on EC 1.14.11.55 - ectoine hydroxylase
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APPLICATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
synthesis
synthesis
efficient conversion of ectoine to hydroxyectoine in Halomonas elongata by heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces anulatus under cotrol of the Hansenula elongata ectA promoter
synthesis
expression of 5-hydroxyectoine biosynthesis enzymes EctA, EctB, EctC, and EctD in Hansenula polymorpha. 5-Hydroxyectoine synthesis in gram per liter scale (2.8 g/l culture supernatant, 365 mol/g dry cell weight) can be achieved, with almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity
synthesis
Stutzerimonas stutzeri
an Escherichia coli cell factory expressing the Pseudomonas stutzeri ectD gene from a synthetic promoter imports homoectoine via the ProU and ProP compatible solute transporters, hydroxylates it, and secretes the formed trans-5-hydroxyhomoectoine, independent from all currently known mechanosensitive channels, into the growth medium
synthesis
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effect of medium formulation (i.e., yeast extract (YE) medium and high yeast extract (HYE) medium) on hydroxyectoine production. Hydroxyectoine production is elevated when the high yeast extract medium is utilized. Hydroxyectoine production increases to 2.9 g/l when 50 mM of 2-oxoglutarate and 1 mM of iron are added to the high yeast extract medium
synthesis
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in an optimized medium containing 100 g/l NaCl in a 500-ml flask, the double mutant lacking EctD and ectoine hydrolaseDoeA synthesizes 3.13 g/l ectoine after 30 h cultivation. Mutants additionally lacking key Na+/H+ antiporter Mrp can synthesize around 7 g/l or 500 mg/(g DCW) in the medium containing lower concentration of NaCl. During a fed-batch fermentation process with 60 g/l NaCl stress, a maximum 10.5 g/l ectoine is accumulated by the Mrp-deficient strain,with a specific production of 765 mg/(g DCW) and a yield of 0.21 g/g monosodium glutamate
synthesis
Stutzerimonas stutzeri A1501
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an Escherichia coli cell factory expressing the Pseudomonas stutzeri ectD gene from a synthetic promoter imports homoectoine via the ProU and ProP compatible solute transporters, hydroxylates it, and secretes the formed trans-5-hydroxyhomoectoine, independent from all currently known mechanosensitive channels, into the growth medium
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synthesis
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in an optimized medium containing 100 g/l NaCl in a 500-ml flask, the double mutant lacking EctD and ectoine hydrolaseDoeA synthesizes 3.13 g/l ectoine after 30 h cultivation. Mutants additionally lacking key Na+/H+ antiporter Mrp can synthesize around 7 g/l or 500 mg/(g DCW) in the medium containing lower concentration of NaCl. During a fed-batch fermentation process with 60 g/l NaCl stress, a maximum 10.5 g/l ectoine is accumulated by the Mrp-deficient strain,with a specific production of 765 mg/(g DCW) and a yield of 0.21 g/g monosodium glutamate
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synthesis
Halomonas salina BCRC 17875
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effect of medium formulation (i.e., yeast extract (YE) medium and high yeast extract (HYE) medium) on hydroxyectoine production. Hydroxyectoine production is elevated when the high yeast extract medium is utilized. Hydroxyectoine production increases to 2.9 g/l when 50 mM of 2-oxoglutarate and 1 mM of iron are added to the high yeast extract medium
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synthesis
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expression of 5-hydroxyectoine biosynthesis enzymes EctA, EctB, EctC, and EctD in Hansenula polymorpha. 5-Hydroxyectoine synthesis in gram per liter scale (2.8 g/l culture supernatant, 365 mol/g dry cell weight) can be achieved, with almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity
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