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1.14.11.55: ectoine hydroxylase

This is an abbreviated version!
For detailed information about ectoine hydroxylase, go to the full flat file.

Wordmap for 1.14.11.55

Word Map on EC 1.14.11.55 Wordmap for 1.14.11.55

Reaction

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ectoine
+
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2-oxoglutarate
+
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O2
=
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5-hydroxyectoine
+
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succinate
+
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CO2

Synonyms

Csal_3003, ectD, EctE, ectoine dioxygenase, HELO_4008, REctD, Sala_2952, SCATT_10620, ThpD

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                EC 1.14.11.551.14.11.55 ectoine hydroxylase

Engineering

Engineering on EC 1.14.11.55 - ectoine hydroxylase

for references in articles please use BRENDA:EC1.14.11.55

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E140A
residue involved in dimerization, activity similar to wild-type
Q127A
residue involved in ectoine binding, about 1% of wild-type activity
R139A
residue involved in dimerization, activity similar to wild-type
R139A/E140A
residues involved in dimerization, activity similar to wild-type
R280A
residue involved in ectoine binding, about 8% of wild-type activity
T149A
residue involved in ectoine binding, about 4% of wild-type activity
W150A
residue involved in ectoine binding, about 2% of wild-type activity
E140A
-
residue involved in dimerization, activity similar to wild-type
-
Q127A
-
residue involved in ectoine binding, about 1% of wild-type activity
-
R139A
-
residue involved in dimerization, activity similar to wild-type
-
R280A
-
residue involved in ectoine binding, about 8% of wild-type activity
-
T149A
-
residue involved in ectoine binding, about 4% of wild-type activity
-
A163C
A163C/S244C
D148A
D148E
F143A
F143W
F143Y
F242A
F242W
F242Y
F263A
F263W
F263Y
H146A
H248A
N133A
N261A
P198A
Q129A
R131A
R259A
R259H
R259K
R259Q
S165A
S167A
S205A
S244C
S250A
V265A
V265L
V265T
W152A
W152F
W152Y