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1.14.11.67: [histone H3]-trimethyl-L-lysine4 demethylase

This is an abbreviated version!
For detailed information about [histone H3]-trimethyl-L-lysine4 demethylase, go to the full flat file.

Word Map on EC 1.14.11.67

Reaction

a [histone H3]-N6,N6,N6-trimethyl-L-lysine4
+ 3 2-oxoglutarate + 3 O2 =
a [histone H3]-L-lysine4
 + 3 succinate + 3 formaldehyde + 3 CO2

Synonyms

amine oxidase (flavin-containing) domain 1, AOF1, At2g34880, CG33182, CG33185, CG3654, dJARID2, dJARID2/CG3654, dJMJD2(1), dJMJD2(1)/CG15835, dJMJD2(2), dJMJD2(2)/CG33182, Fbxl10, GH09982, H3K4 demethylase, H3K4 demethylase lysine-specific demethylase 5A, H3K4 histone demethylase, H3K4me3 demethylase, H3K4me3 histone demethylase, H3K4me3-specific demethylase, H3K4me3/2 histone demethylase, H3K9 trimethyl demethylase, histone 3 lysine 4 demethylase, histone demethylase, histone H3 lysine 4 demethylase, histone H3 lysine-4 demethylase, histone H3-K4 demethylase, histone H3K4 demethylase, histone H3K4-specific demethylase, histone lysine demethylase, histone-H3K4-specific demethylase, Jarid1, Jarid1a, JARID1B, JARID1C, Jarid2, JaridB, Jhd2, Jhdm1b/Kdm2b, JMJ15, JMJ703, JmjC domain histone demethylase, JmjC domain-containing histone demethylation protein 3A, JmjC domain-containing histone demethylation protein 3b, JmjC+N, JmjC+N histone demethylase, JMJD2A, JMJD2A-tudor, JMJD2B, jumonji AT rich interactive domain 1B, jumonji AT-rich interactive domain 1B, Jumonji demethylase, KDM1, KDM1B, KDM2, KDM5, KDM5A, KDM5B, KDM5b/JARID1b, KDM5C, KDM5D, Lid, little imaginal discs, LSD1, lysine demethylase, lysine demethylase 5b, lysine demethylase 7, lysine-specific demethylase, lysine-specific demethylase 1, lysine-specific demethylase 5A, lysine-specific demethylase 5B, lysine-specific demethylase JMJ703, Lysine-specific demethylase SE14, More, Ndy1, Os03g0151300, PHD finger protein 8, PHF8, PKDM7B, PLU-1, PLU1, RB-binding protein 2, RBP2, RBR-2, retinoblastoma binding protein 2, SE14, SMCX, trimethylated lysine 4 of histone H3 demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.67 [histone H3]-trimethyl-L-lysine4 demethylase

Cloned

Cloned on EC 1.14.11.67 - [histone H3]-trimethyl-L-lysine4 demethylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding for Myc-tagged JARID1B is transfected into HeLa cells, and the effect on histone lysine methylation is examined
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co-expression of FLAG-CMV-tagged hPc2 and the HA-tagged N-terminal fragment of JARDI1B in HEK-293T cells. Expression the His-tagged N-terminal fragment of JARID1B in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
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expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in HEK-293T cells and NIH-3T3 cells
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expressed in Sf21 insect cells
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expressed in Sf9 insect cells
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full-length Flag-tagged human PLU-1 is expressed in SF9 cells. PLU-1 is overexpressed in U2OS cells
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gene CG3654, sequence comparisons, recombinant overexpression of FLAG-tagged enzyme in S2 cells. Overexpression experiments, performed both in S2 cells and flies, fail to detect any demethylase activity of dJARID2/CG3654 on H3K4me3, H3K9me3, H3K27me3, H3K36me3 and H4K20me3
gene JHD2, recombinant expression of HA-tagged wild-type Jhd2 and H427A mutant in yeast cells
gene JMJ15, quantitative RT-PCR enzyme expression analysis, pJMJ15-GUS and 35S-JMJ15-FLAG-HA constructs are transformed into Agrobacterium tumefaciens strain GV3101 and then transformed into plants using the floral dip method
gene JMJ703, DNA and amino acid sequence determination and analysis, genotyping, functional overexpression of YFP-HA-tagged JMJ703 in Nicotiana tabacum cells via transfection with Agrobacterium tumefaciens strain EHA105
gene JMJ703, functional transient overexpression of the FLAG:HA-tagged JmjN-JmjC-zinc finger region in Nicotiana tabacum leaf nuclei
gene KDM1A, real-time PCR enzyme expression analysis
gene KDM5A, cloned from a HeLa gene library, recombinant expression of N-terminally FLAG-tagged KDM5A in HeLa cells and HEK-293T cells, quantitative RT-PCR expression analysis
gene KDM5A, recombinant coexpression of wild-type and mutant FLAG-tagged Piasy and HA-tagged Rbp2 from pcDNA3.1(+) HA expression vector and p33 Flag CMV7.1 expression vector, respectively, in HEK-293T cells
gene KDM5A, recombinant expression of soluble His-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3)C+
gene KDM5B, recombinant expression of soluble His-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3)C+
gene Kdm5c, encoded on the X-chromosome, genotyping, quantitative real-time RT-PCR expression analysis
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gene KDM5C, recombinant expression ofsoluble His-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3)C+
gene rbr2, generation of transgenic lines in which RBR-2::GFP expression is driven by the rbr-2 promoter showing that RBR-2 is ubiquitously expressed in embryos and larvae, gene rbr-2 interacts with many neuronal genes, overview
gene SE14, map-based cloning of Se14, DNA and amino acid sequence determination and analysis, sequence comparisons, fine mapping of the Se14 locus on the short arm of chromosome 3, genetic interaction of the Se14 locus with other flowering time genes loci and gene expression analysis
overexpression of FLAG-tagged CG15835 in S2 cells
overexpression of Flag-tagged Mrg15, CG13367, and Lid in S2 cells
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overexpression of KDM5b in embryonic stem cells as Flag-tagged and EGFP-tagged protein. Constitutive expression of KDM5b modifies embryonic stem cell proliferation and 3meH3K4 levels. Quantitative RT-PCR expression analysis
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RBP2, gene expression profiling and computational analyses of gene targeting
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recombinant Ndy1 overexpression in mouse embryonic fibroblasts, quantitative real-time reverse transcriptase PCR expression analysis
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stable expression of Flag-tagged KDM5B in HEK-293 cells
stable overexpression of N-terminally HA-tagged Fbxl10 in murine embryonic fibroblasts leading to an increase in cell and nuclear size and changes the transcriptome, but with no effect on proliferation, mitosis, and apoptosis or global histone marks, quantitative real-time PCR expression analysis
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