co-expression of FLAG-CMV-tagged hPc2 and the HA-tagged N-terminal fragment of JARDI1B in HEK-293T cells. Expression the His-tagged N-terminal fragment of JARID1B in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
gene CG3654, sequence comparisons, recombinant overexpression of FLAG-tagged enzyme in S2 cells. Overexpression experiments, performed both in S2 cells and flies, fail to detect any demethylase activity of dJARID2/CG3654 on H3K4me3, H3K9me3, H3K27me3, H3K36me3 and H4K20me3
gene JMJ15, quantitative RT-PCR enzyme expression analysis, pJMJ15-GUS and 35S-JMJ15-FLAG-HA constructs are transformed into Agrobacterium tumefaciens strain GV3101 and then transformed into plants using the floral dip method
gene JMJ703, DNA and amino acid sequence determination and analysis, genotyping, functional overexpression of YFP-HA-tagged JMJ703 in Nicotiana tabacum cells via transfection with Agrobacterium tumefaciens strain EHA105
gene KDM5A, cloned from a HeLa gene library, recombinant expression of N-terminally FLAG-tagged KDM5A in HeLa cells and HEK-293T cells, quantitative RT-PCR expression analysis
gene KDM5A, recombinant coexpression of wild-type and mutant FLAG-tagged Piasy and HA-tagged Rbp2 from pcDNA3.1(+) HA expression vector and p33 Flag CMV7.1 expression vector, respectively, in HEK-293T cells
gene rbr2, generation of transgenic lines in which RBR-2::GFP expression is driven by the rbr-2 promoter showing that RBR-2 is ubiquitously expressed in embryos and larvae, gene rbr-2 interacts with many neuronal genes, overview
gene SE14, map-based cloning of Se14, DNA and amino acid sequence determination and analysis, sequence comparisons, fine mapping of the Se14 locus on the short arm of chromosome 3, genetic interaction of the Se14 locus with other flowering time genes loci and gene expression analysis
overexpression of KDM5b in embryonic stem cells as Flag-tagged and EGFP-tagged protein. Constitutive expression of KDM5b modifies embryonic stem cell proliferation and 3meH3K4 levels. Quantitative RT-PCR expression analysis
stable overexpression of N-terminally HA-tagged Fbxl10 in murine embryonic fibroblasts leading to an increase in cell and nuclear size and changes the transcriptome, but with no effect on proliferation, mitosis, and apoptosis or global histone marks, quantitative real-time PCR expression analysis