1.14.11.8: trimethyllysine dioxygenase
This is an abbreviated version!
For detailed information about trimethyllysine dioxygenase, go to the full flat file.
Word Map on EC 1.14.11.8
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1.14.11.8
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carnitine
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gamma-butyrobetaine
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autism
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inborn
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proliferator-activated
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hydroxymethyltransferase
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transporter-2
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aldolase
- 1.14.11.8
- carnitine
- gamma-butyrobetaine
-
autism
-
inborn
-
proliferator-activated
- hydroxymethyltransferase
-
transporter-2
- aldolase
Reaction
Synonyms
6-N-trimethyllysine dioxygenase, epsilon-N-trimethyllysine hydroxylase, epsilon-trimethyllysine 2-oxoglutarate dioxygenase, eta-N-trimethyllysine hydroxylase, Nepsilon-trimethyllysine hydroxylase, oxygenase, trimethyllysine di-, TML dioxygenase, TML hydroxylase, TML-alpha-ketoglutarate dioxygenase, TMLD, TMLH, TMLH-a, TMLH-b, trimethyllysine alpha-ketoglutarate dioxygenase, trimethyllysine hydroxylase
ECTree
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Purification
Purification on EC 1.14.11.8 - trimethyllysine dioxygenase
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recombinant active and stable His- and MBP-tagged TMLH from Escherichia coli strain BL21-AI by nickel or amylose affinity chromatography. It is possible that the His6-tag at the N-terminus of the fusion protein is masked and cannot be accessed by the Ni-beads. Thus, MBP affinity purification is selected as a first purification step, followed by gel filtration, His6-MBP-tag cleavage by recombinant TEV protease
recombinant MBP-taged enzyme by affinity chromatography, tag cleavage by rTEV protease, and gel filtration